Journal of Pharmacy And Bioallied Sciences
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REVIEW ARTICLE
Year : 2012  |  Volume : 4  |  Issue : 4  |  Page : 267-275

HybridSPE: A novel technique to reduce phospholipid-based matrix effect in LC-ESI-MS Bioanalysis


1 School of Pharmacy and Technology Management, NMIMS University, Mumbai, India
2 Department of Bioanalytical, AshcoNeulab Ltd, Mumbai, India
3 Faculty of Pharmacy, Integral University, Lucknow, India

Correspondence Address:
Md Akhlaquer Rahman
Faculty of Pharmacy, Integral University, Lucknow
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0975-7406.103234

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When complex biological materials are analyzed without an adequate sample preparation technique, MS signal and response undergo significant alteration and result in poor quantification and assay. This problem generally takes place due to the presence of several endogenous materials component in samples. One of the major causes of ion suppression in bioanalysis is the presence of phospholipids during LC-MS analysis. The phospholipid-based matrix effect was investigated with a commercially available electro spray ionization (ESI) source coupled with a triple quadrupole mass spectrometer. HybridSPE dramatically reduced the levels of residual phospholipids in biological samples, leading to significant reduction in matrix effects. This new procedure that combines the simplicity of precipitation with the selectivity of SPE allows obtaining much cleaner extracts than with conventional procedures. HybridSPE-precipitation procedure provides significant improvement in bioanalysis and a practical and fast way to ensure the avoidance of phospholipids-based matrix effects. The present review outlines the HybridSPE technique to minimize phospholipids-based matrix effects on LC-ESI-MS bioanalysis.


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