Journal of Pharmacy And Bioallied Sciences
Journal of Pharmacy And Bioallied Sciences Login  | Users Online: 3400  Print this pageEmail this pageSmall font sizeDefault font sizeIncrease font size 
    Home | About us | Editorial board | Search | Ahead of print | Current Issue | Past Issues | Instructions | Online submission




 
 Table of Contents  
ORIGINAL/BRIEF
Year : 2012  |  Volume : 4  |  Issue : 5  |  Page : 60-61  

Preparation and formulation of transferosomes containing an antifungal agent for transdermal delivery: Application of Plackett-Burman design to identify significant factors influencing vesicle size


Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Changa, Gujarat, India

Date of Web Publication21-Mar-2012

Correspondence Address:
B Rutu Patel
Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Changa, Gujarat
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0975-7406.94140

Rights and Permissions
   Abstract 

Transferosomes containing an anti-fungal agent were prepared by Rotary Flask Evaporation -Sonication method. Eight batches were prepared in triplicate and vesicle size of each batch was determined. Plackett-Burman Design was employed to identify significant formulation and process parameters affecting vesicle size. The amount of lipid and surfactant, volume of ethanol and hydration medium as well as hydration time significantly affect the vesicle size.

Keywords: Transferosomes, ultradeformable vesicles, vesicle size


How to cite this article:
Patel B R, Parikh R H. Preparation and formulation of transferosomes containing an antifungal agent for transdermal delivery: Application of Plackett-Burman design to identify significant factors influencing vesicle size. J Pharm Bioall Sci 2012;4, Suppl S1:60-1

How to cite this URL:
Patel B R, Parikh R H. Preparation and formulation of transferosomes containing an antifungal agent for transdermal delivery: Application of Plackett-Burman design to identify significant factors influencing vesicle size. J Pharm Bioall Sci [serial online] 2012 [cited 2020 Jan 27];4, Suppl S1:60-1. Available from: http://www.jpbsonline.org/text.asp?2012/4/5/60/94140

Transferosomes are also known as Ultradeformable vesicles, Ultradeformable Liposomes or Elastic Vesicles. Transfersome can squeeze itself through a skin pore which is many times smaller than its size owing to its elasticity. Thus, they can increase the skin penetration when applied non-occlusively. The objective of the present study was to identify significant factors influencing vesicle size through the use of Plackett-Burman Design.


   Materials and Methods Top


Preparation of transferosomes

They were prepared by Rotary Flask Evaporation -Sonication Method reported by Gregor et al. [1] Soya phosphatidyl choline, Tween80 and antifungal agent were dissolved in Ethanol. Ethanol was evaporated by flask rotation to deposit the film on the wall of the round bottom flask. The film was hydrated at 45°C using Saline phosphate buffer, pH 6.4 with mild agitation. The Transferosomes formed were allowed to swell for 2 hour at room temperature. Vesicles were subsequently sonicated for 30minutes using bath sonicator. (Model: EN-60US,Make: ELECTROQUIP). Eight batches were prepared as per the Plackett - Burman Design [Table 1].
Table 1: Composition of transferosomes of different batches and their vesicle size

Click here to view


Measurement of vesicle size

Vesicle size was determined using particle size analyzer employing Dynamic Light Scattering Technique (Model: Nano S90, Make: Malvern, USA). Data analysis was carried out using DOE ++ Software (Version 1.0.7.2 ID -DS-1, ReliaSoft Corporation, USA).


   Result and Discussion Top


Using Pareto chart [Figure 1] significant and non-significant factors influencing vesicle size were identified. It is evident from the Pareto Chart [Figure 1] as well as P value [Table 1] with respect to vesicle size associated with every variable that the amount of Lipid, amount of Surfactant, volume of Ethanol, volume of Hydration medium and Hydration Time significantly affect the vesicle size.
Figure 1: Pareto chart showing effects of variables on vesicle size

Click here to view



   Conclusion Top


From large number of formulation and process variables involved in making of transferosomes, Plackett - Burman Design can help in identifying significant factors influencing vesicle size. These significant factors may be optimized by employing full factorial design. While employing full factorial design, non-significant factors may be fixed at appropriate level (-1 or +1) employed during Plackett - Burman Design.


   Acknowledgement Top


We are very thankful to Halcyon Labs Pvt. Ltd., Ahmedabad for providing an ANTIFUNGAL AGENT as a gratis sample.[7]

 
   References Top

1.Gregor C. Rational design of new product candidates: The next generation of highly deformable bilayer vesicles for noninvasive, targeted therapy. Journal of Controlled Release 2012; Article in press.  Back to cited text no. 1
    
2.Hardevinder Pal Singh, P. U., Ashok Kumar Tiwary,and Subheet Jain, Elastic Liposomal Formulation for Sustained Delivery of Colchicine: in vitro Characterization and in vivo Evaluation of Anti-gout Activity. The AAPS Journal 2009: 11: 54-64.  Back to cited text no. 2
    
3.P. Loan Honeywell-Nguyen, J. A. B., T he in vitro transport of pergolide from surfactant-based elastic vesicles through human skin: A suggested mechanism of action. Journal of Controlled Release 2003: 86: 145-156.  Back to cited text no. 3
    
4.A. Gillet, A. G., P. Compèrec, B. Evrarda, G. Piel Development of a new topical system: Drug-in-cyclodextrin-in-deformable liposome. International Journal of Pharmaceutics 2009: 380: 174-180.  Back to cited text no. 4
    
5.Gamal M.M. El Maghraby, A. C. W., Brian W. Barry, Oestradiol skin delivery from ultradeformable liposomes: refinement of surfactant concentration. International Journal of Pharmaceutics 2000: 196: 63-74.  Back to cited text no. 5
    
6.Ghada M. El Zaafarany, G. A. S. A., Samar M. Holayel, Nahed D. Mortada, Role of edge activators and surface charge in developing ultradeformable vesicles with enhanced skin delivery. International Journal of Pharmaceutics 2010: 397: 164-172.  Back to cited text no. 6
    
7.Gregor Cevc, G. B., New, highly efficient formulation of diclofenac for the topical,transdermal administration in ultradeformable drug carriers,Transfersomes. Biochimica et Biophysica Acta, 2001:1514:191-205.  Back to cited text no. 7
    


    Figures

  [Figure 1]
 
 
    Tables

  [Table 1]


This article has been cited by
1 Nanotechnological approaches for the effective management of psoriasis
Tarun Garg,Goutam Rath,Amit K. Goyal
Artificial Cells, Nanomedicine, and Biotechnology. 2015; : 1
[Pubmed] | [DOI]
2 Recent advances in permeation enhancement techniques for Transdermal drug delivery systems: A review
Kamboj, S., Jhawat, V., Saini, V., Bala, S.
Current Drug Therapy. 2013; 8(3): 181-188
[Pubmed]



 

Top
 
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

 
  In this article
    Abstract
    Materials and Me...
    Result and Discu...
   Conclusion
   Acknowledgement
    References
    Article Figures
    Article Tables

 Article Access Statistics
    Viewed1669    
    Printed78    
    Emailed1    
    PDF Downloaded135    
    Comments [Add]    
    Cited by others 2    

Recommend this journal