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ORIGINAL ARTICLE
Year : 2013  |  Volume : 5  |  Issue : 1  |  Page : 61-65

A validated RP-HPLC method for simultaneous determination of propranolol and valsartan in bulk drug and gel formulation


1 Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, New Delhi, India
2 Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia

Correspondence Address:
Mohammed Aqil
Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, New Delhi
India
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Source of Support: All India Council for Technical Education (AICTE), New Delhi, (Grant # 8023/BOR/RPS.157/2006.07), Conflict of Interest: None


DOI: 10.4103/0975-7406.106573

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Objective: A simple, precise, and stability indicating high performance liquid chromatography (HPLC) method was developed and validated for the simultaneous determination of propranolol hydrochloride and valsartan in pharmaceutical dosage form. Materials and Methods: The method involves the use of easily available inexpensive laboratory reagents. The separation was achieved on Hypersil ODS C-18 column (250*4.6 mm, i.d., 5 μm particle size) with isocratic flow with UV detector. The mobile phase at a flow rate of 1.0 mL/min consisted of acetonitrile, methanol, and 0.01 M disodium hydrogen phosphate (pH 3.5) in the ratio of 50:35:15 v/v. Results: A linear response was observed over the concentration range 5-50 μg/mL of propranolol and the concentration range 4-32 μg/mL of valsartan. Limit of detection and limit of quantitation for propranolol were 0.27 μg/mL and 0.85 μg/mL, and for valsartan were 0.45 μg/mL and 1.39 μg/mL, respectively. The method was successfully validated in accordance to ICH guidelines acceptance criteria for linearity, accuracy, precision, specificity, robustness. Conclusion: The analysis concluded that the method was selective for simultaneous estimation of propranolol and valsartan can be potentially used for the estimation of these drugs in combined dosage form.


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