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ORIGINAL ARTICLE
Year : 2013  |  Volume : 5  |  Issue : 3  |  Page : 214-223

In vitro antioxidant and H + , K + -ATPase inhibition activities of Acalypha wilkesiana foliage extract


1 Department of PG Studies and Research in Biotechnology and Bioinformatics, Jnanasahyadri, Kuvempu University, Shankaraghatta, India
2 Department of Biotechnology, NMAM Institute of Technology, Nitte, Karkala, Udupi, Karnataka, India

Correspondence Address:
Manjunatha Hanumanthappa
Department of PG Studies and Research in Biotechnology and Bioinformatics, Jnanasahyadri, Kuvempu University, Shankaraghatta
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0975-7406.116822

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Aims: The aim of this study was to evaluate the antioxidant activty and anti-acid property of Acalypha wilkesiana foliage extract. Materials and Methods: Hot and cold aqueous extracts were prepared from healthy leaves of A. wilkesiana. Free radical scavenging activity and H + , K + -ATPase inhibition activities of aqueous foliage extracts was screened by in vitro models. Statistical Analysis Used: All experiments were performed in triplicate and results are expressed as mean ± SEM. Results: A. wilkesiana hot aqueous extract (AWHE) showed significant antioxidants and free radical scavenging activity. Further, AWHE has shown a potent H + , K + -ATPase inhibitory activity (IC 50 : 51.5 ± 0.28 μg/ml) when compare to standard proton pump inhibitor omeprazole (56.2 ± 0.64 μg/ml); however, latter activity is equal to A. wilkesiana cold aqueous extract (AWCE). Quantitative analysis of AWHE has revealed more content of phenols and flavonoids; this is found to be the reason for good antioxidant activity over AWCE. Molecular docking was carried out against H + , K + -ATPase enzyme crystal structure to validate the anti-acid activity of A. wilkesiana major phytochemicals. Conclusions: The present study indicates that the constituents of AWHE and AWCE have good antacid and free radical scavenging activity.


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