Journal of Pharmacy And Bioallied Sciences
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ORIGINAL ARTICLE
Year : 2017  |  Volume : 9  |  Issue : 1  |  Page : 1-7

Estimation of guggulsterone E and Z in the Guggul-based commercial formulations using high-performance thin-layer chromatography


1 Centre of Excellence in Biotechnology, M. P. Council of Science and Technology, Bhopal, Madhya Pradesh, India
2 Department of Postgraduate Studies and Research in Biological Science, Bacteriology Laboratory, Rani Durgavati University, Jabalpur, Madhya Pradesh, India
3 Madhya Pradesh Pollution Control Board, Paryavaran Parisar, Bhopal, Madhya Pradesh, India

Correspondence Address:
Pramod Kumar Sairkar
Centre of Excellence in Biotechnology, M.P. Council of Science and Technology, Vigyan Bhavan, Nehru Nagar, Bhopal, Madhya Pradesh
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0975-7406.206225

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Background: Guggulsterone(GS) is a plant steroid and bioactive compound present in gum Guggul of Commiphora wightii. An Indian herbal medicine system “Ayurveda” has a long history of use of gum Guggul and plant extract of C. wightii as medicine for the treatment of various illnesses. Complex nature, low availability, and inconsistency of phytoconstituents make its analysis of difficult tasks. Aims: In this work, six different Guggul-based herbal formulations were examined for estimation of GS and their isomers(E and Z) through high-performance thin-layer chromatography technique. Materials and Methods: For that various concentrations of standard E-GS and Z-GS(50 ng–250ng/spot) with samples (20 μg/spot) were applied on silica gel coated aluminum plate and developed with the mobile phase of toluene: ethyl acetate: formic acid: methanol(6:2:1:0.5). The scanning was performed at 254nm wavelength and the absorbance(scan) spectrum of E-GS and Z-GS peak was generated at 200 nm–400nm wavelength range. Results and Conclusions: Rf value and scan spectrum pattern of the samples reveal that they contain either one form of GS(E-GS, Z-GS) or both. The quantity of E-GS and Z-GS within the samples was ranged from 0.230±0.0040–0.926±0.0168% to 0.537±0.0026–0.723±0.0177%, respectively.


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