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ORIGINAL ARTICLE
Year : 2018  |  Volume : 10  |  Issue : 3  |  Page : 137-143

Potential deoxycytidine kinase inhibitory activity of amaryllidaceae alkaloids: An in silico approach


1 Department of Pharmacology, Faculty of Pharmacy, University of Khartoum; Department of Pharmacology, Faculty of Pharmacy, Sudan International University, Khartoum, Sudan
2 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum; Medicinal, Aromatic Plants and Traditional Medicine Research Institute, National Centre for Research, Khartoum, Sudan
3 Department of Pharmacology, Faculty of Pharmacy, University of Khartoum, Khartoum, Sudan
4 Department of Pharmacology and Toxicology, College of Pharmacy, University of Hail, Hail, Saudi Arabia
5 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum, Khartoum, Sudan

Correspondence Address:
Mrs. Amina I Dirar
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum, PO Box 1996, Khartoum
Sudan
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/JPBS.JPBS_44_18

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Background: Plants of the Amaryllidaceae family have been under intense scrutiny for the presence of a couple of alkaloidal secondary metabolites with endued cytotoxic activity, such as pancratistatin (1), 7-deoxypancratistatin (2), narciclasine (3), 7-deoxynarciclasine (4), trans-dihydronarciclasine (5), and 7-deoxy-trans-dihydronarciclasine (6). Nevertheless, preclinical evaluation of these alkaloids has been put on hold because of the limited quantity of materials available from isolation. Aim: To explore the underlying cytotoxic molecular mechanisms of the Amaryllidaceae alkaloids (1–6) and to assess their absorption, distribution, metabolism, excretion, and toxicity (ADMET) profiles using chemoinformatic tools. Materials And Methods: AutoDock 4.0 software along with different in silico chemoinformatic tools, namely PharmMapper, Molinspiration, MetaPrint2D, and admetSAR servers, were used to assess the drugability of the Amaryllidaceae alkaloids (1–6). Results: Deoxycytidine kinase (dCK) (PDB: 1P60) was predicted as a potential target with fitting score of 5.574. In silico molecular docking of (1–6) into dCK revealed good interactions, where interesting hydrogen bonds were observed with the amino acid residues—Gly-28 and Ser-35—located in the highly conserved P-loop motif. This motif plays a special role in dCK function. Contrary to (1), in silico pharmacokinetic results have shown good absorption and permeation and thus good oral bioavailability for (2–6). Conclusion: The in silico docking data have proposed that the reported cytotoxic activity of the Amaryllidaceae alkaloids (1–6) could be mediated through dCK inhibition. In addition, the ADMET profile of these alkaloids is promising and thus (1–6) could be candidates for future drug development.


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