|Year : 2020 | Volume
| Issue : 5 | Page : 114-123
Surface characteristics and bioactivity of zirconia (Y-TZP) with different surface treatments
Gayathree Alagiriswamy, Chitra Shankar Krishnan, Hariharan Ramakrishnan, Sampath Kumar Jayakrishnakumar, Vallabh Mahadevan, Nagarasampatti Sivaprakasam Azhagarasan
Department of Prosthodontics & Implantology, Ragas Dental College & Hospital, Chennai, Tamil Nadu, India
|Date of Submission||25-Jan-2020|
|Date of Acceptance||02-Mar-2020|
|Date of Web Publication||25-Aug-2020|
Department of Prosthodontics & Implantology, Ragas Dental College & Hospital, Uthandi, Chennai 600119, Tamil Nadu
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Zirconia being a bio-inert material needs to be surface treated to render it more bioactive and enhance its osseointegration potential. However, bioactivity studies focusing on the ability of sandblasting and ultraviolet photofunctionalization (UVP) surface treatments in inducing apatite precipitation using simulated body fluid (SBF) are lacking. Aim: The aim of the study was to comparatively evaluate the effect of two different surface treatments—sandblasting with 50 µm alumina and UVP with ultraviolet C (UVC) light on the bioactivity of zirconia. Materials and Methods: A total of 33 discs with dimensions 10 mm × 2 mm were obtained from zirconia blanks (Amann Girrbach, Koblach, Austria) and randomly divided into three groups (n = 11), namely Group I (untreated), Group II (sandblasted), and Group III (UVP). Surface characteristics of representative test samples were analyzed using X-ray diffraction (XRD), atomic force microscopy (AFM), contact angle goniometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDX), to assess type of crystal phase of zirconia, surface roughness, wettability, surface topography, and elemental composition, respectively. SBF was prepared and calcium content in SBF (Ca-SBF) was determined using inductively coupled plasma mass spectrometry (ICP-MS). Results: Data were analyzed by one-way analysis of variance (ANOVA), post hoc Tukey honestly significant difference (HSD), and Student’s t test for statistical significance (P < 0.05, significant; P < 0.01, highly significant). Surface characteristics analyses revealed that XRD showed predominant tetragonal (t) zirconia crystal phase for all test groups. Mean surface roughness (Sa) of Group I was 41.83 nm, and it was significantly lesser than that of Group II (115.65 nm) and Group III (102.43 nm). Mean contact angles were 98.26°, 86.77°, and 68.03° for Groups I, II, and III, respectively, and these differences were highly significant. Mean pre-immersion Ca content in SBF was found to be 159 mg/L. Mean post-immersion Ca content was 70.10, 60.80, and 56.20 mg/L for Groups I, II, and III, respectively. Significant differences were found between Group I as compared to both Groups II and III. Bioactivity of Group III was marginally, but insignificantly higher with respect to Group II. Groups II and III were insignificant with respect to each other. Post-immersion XRD revealed predominant “t” phase, and SEM-EDX revealed well-formed, abundant calcium apatite layer on the treated samples as compared to that on untreated sample and an increasing Ca/P ratio from 1.15, 1.79 to 2.08, respectively from Group I to Group III. Conclusion: Within the limitations of this study, both sandblasting and UVP significantly and similarly improved bioactivity of zirconia as compared to the untreated samples, which was corroborated by the SEM-EDX results.
Keywords: Bioactivity, sandblasting, simulated body fluid, surface treatment, ultraviolet photofunctionalization, zirconia
|How to cite this article:|
Alagiriswamy G, Krishnan CS, Ramakrishnan H, Jayakrishnakumar SK, Mahadevan V, Azhagarasan NS. Surface characteristics and bioactivity of zirconia (Y-TZP) with different surface treatments. J Pharm Bioall Sci 2020;12, Suppl S1:114-23
|How to cite this URL:|
Alagiriswamy G, Krishnan CS, Ramakrishnan H, Jayakrishnakumar SK, Mahadevan V, Azhagarasan NS. Surface characteristics and bioactivity of zirconia (Y-TZP) with different surface treatments. J Pharm Bioall Sci [serial online] 2020 [cited 2020 Sep 23];12, Suppl S1:114-23. Available from: http://www.jpbsonline.org/text.asp?2020/12/5/114/292866
| Introduction|| |
Developments in clinical prosthodontics are driven by the innovation of new dental materials and processing techniques. The research in implant biomaterials is surging since past few decades due to a continuous increase in the aging population, who demand increasingly functional and aesthetic prosthodontic replacements. The criteria for a restorative material to be termed as a “biomaterial” is that it has to be biocompatible with excellent aesthetic and mechanical properties., Currently, yttria-stabilized zirconia, also known as tetragonal zirconia polycrystal (Y-TZP), is the most used material for fabrication of zirconia-based implants.
Despite addition of stabilizing elements, zirconia is a bio-inert material,,,, and this aspect may impact its osseointegration potential.,, Hence, studies focusing on surface treatments of zirconia to render the surface more receptive to osseointegration and apatite formation have gained significance., However, t-m phase conversions after certain surface treatments that can deleteriously affect the longevity of zirconia as an implant biomaterial have also been reported, and hence ascertaining maintenance of the tetragonal phase following any type of surface treatment of zirconia is crucial in bioactivity studies. Various reports are available stating the importance of surface topography and characteristics, such as surface roughness and wettability on the extent of bioactivity of zirconia,,, following different surface treatments. Wettability has been suggested as a key parameter that impacts the chain of processes associated with osseointegration.,, The surface topography and elemental composition is also thought to influence the maintenance of the tetragonal phase as well as affects its bioactivity. Thus, bioactivity studies also typically include surface characteristics investigations comparing untreated and treated zirconia surfaces to explain the bioactivity.,, Methods such as X-ray diffraction (XRD), atomic force microscopy (AFM), contact angle goniometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDX) are used by researchers to assess crystal phase, roughness, wettability, topography, and elemental composition, respectively.,,,,
Several reports have summarized different additive and subtractive surface modification methods to improve surface properties of zirconia implant biomaterials. These reports found improved bone bonding, achieved due to surface treatments when compared to untreated surfaces.,
Airborne particle abrasion known as sandblasting technique has been used to increase surface roughness of zirconia, which has been shown to positively impact osseointegration in cell culture studies.,,, One concern that is often mentioned is that sandblasting could result in damage to the zirconia surface, thereby altering the vital surface characteristics. Airborne particle abrasion with alumina particles lesser than 100 µm in size has been identified as a key factor in achieving an optimum surface roughness to enhance biological response of osteoblasts without causing structural damage to zirconia.,,,,,
Recently, researchers have reported that ultraviolet photofunctionalization (UVP) as a surface modification of zirconia enhances its osseointegration potential.,,, “Bioactivity” is one of the characteristics of an implant material, which allows it to form a bond with living tissue.,In vitro testing such as simulated body fluid (SBF) analysis and protein adsorption assays have been used to mimic in vivo conditions, thereby decreasing time, cost, and regulatory issues, and it can be manipulated by researchers in a controlled manner.,,
Studies have recommended the use of in vitro bioactivity tests such as immersion of synthetic materials into solutions such as SBF, which replicate the mineral content of human plasma., The calcium and phosphorous content in SBF form apatite precipitation on these biomaterials to varying extents, depending on the material, their surface characteristics, duration of immersion environment, and so on.,,, Thus, immersion in SBF can aid to predict in vivo behavior of a potential implant biomaterial. In vitro testing of bioactivity in SBF has also minimized the requirement of animal studies.,,,
Calcium content analysis of the SBF solution by inductively coupled plasma mass spectrometry (ICP-MS) both before and after immersion of samples has been recommended as a reliable method to assess the apatite precipitation, which indicates its bioactivity., Cell culture and protein adsorption studies exploring the bioactive potential of sandblasting and UVP surface treatments on zirconia are available in the literature.,,,,,,
Thus, studying these characteristics aids in the correlation of bioactivity results and are frequently used as an adjunct in such studies.,,,,,
| Materials and Methods|| |
Commercially available Y-TZP (Amann Girrbach, Koblach, Austria) was used.
Customizing test samples
Thirty zirconia samples with dimensions of thickness 10 mm × 2 mm diameter with extension tag of 4 × 4 × 2 mm were obtained from copy-milling a customized resin prototype and then sintering it at 1500°C for 8 h.
Grouping of samples and surface treatment
The above processed zirconia were divided and designated as Groups I, II, and III depending on the type of surface treatment (n = 10/group):
- Group I (untreated): Samples (n = 10) that were not subjected to any surface treatment
- Group II (sandblasted): Samples (n = 10) that were subjected to sandblasting with 50 µm alumina at a distance of 10 mm/at an angle of 90°/and at 2.5 bar pressure for 15s followed by ultrasonic cleaning for 15 min in de-ionized water to remove alumina particles
- Group III (UVP): Samples (n = 10) that were placed in a UV chamber at a distance of 4cm from the light source of wavelength 254 nm for 15 min
All the samples of the three test groups were subsequently marked, dried, and stored in a desiccator (Borosil, Mumbai, India) until further testing.
Surface characteristic analysis
X-ray diffractometer (XRD) (Ultima IV, Rigaku, Tokyo, Japan) was used to detect the type of crystal phase present on representative samples (n = 1/group) of all groups.
Atomic force microscopy
AFM was used to detect surface roughness of representative samples of untreated and treated test groups. The test samples were analyzed in four areas of each sample of each group, by keeping the cantilever tip of the equipment in noncontact mode. Subsequently, 5 µm × 5 µm images with 256 × 256 pixels were taken with a scan rate of 0.5 Hz, to obtain both two- (2D) and three-dimensional (3D) images. The analysis also gave numeric values for surface roughness (Sa) at nanoscale levels for each group.
Contact angle goniometry
The surface wettability (hydrophilicity) of representative samples (n = 5/group) of all three groups was examined by static sessile-drop technique using 1 µL H2O by Universal Goniometer DSA 20E (Kruss, Hamburg, Germany). Five samples per test group were analyzed by measuring five contact angle values per sample, and the overall mean contact angle was recorded.
Scanning electron microscopy coupled with energy dispersive X-ray spectroscopy
The surface topography and surface elemental composition of representative samples (n = 1/group) was analyzed by field emission SEM (Thermo Fisher Scientific, Waltham, Massachusetts) coupled with EDX (Bruker, Billerica, Massachusetts).
The samples were gold-coated using auto-fine coaters and observed with scanning electron microscope (SEM) at ×5000.
The samples of all three groups (n = 10/group) were immersed in SBF prepared according to Kokubo and Takadama for 3 weeks (21 days) at 36.5°C.
After ultrasonic washing and rinsing with de-ionized water, zirconia samples were immersed in SBF at 36.5°C without stirring to investigate the bioactivity. The SBF was prepared according to the method proposed by Kokubo and Takadama. The SBF was freshly prepared four times, and the mean pre-immersion calcium content was determined using ICP-MS. After immersion for 21 days, the samples were removed, rinsed with de-ionized water, and dried in a desiccator. The calcium content of SBF solution from each test tube containing samples was assessed by ICP-MS.
| Results|| |
Atomic force microscopy
The 3D image of representative sample of Group I (untreated) surface revealed a uniform appearance throughout with lesser number of shallow peaks and valleys, seen at isolated places on the sample surface. The average height of peaks and valleys was found to be 292.13 nm. The 3D image of representative sample of Group II (sandblasted) surface revealed a nonuniform texture, with greater number of very high and well-defined peaks and valleys distributed throughout the sample surface. The average height of peaks and valleys was 764.06 nm, showing the effect of surface treatment. The 3D image of representative sample of Group III (UVP) surface revealed a nonuniform texture of roughened plains with several deep grooves, along with areas of clustered peaks and valleys, which were well-defined. The average height of peaks and valleys was 541.65 nm. The effect of treatment was reflected in their patterns.
Sandblasting and UV irradiation significantly modified surface roughness (P < 0.01). One-way ANOVA revealed overall significant difference between the mean surface roughness values of the three groups (Groups I, II, and III) [Table 1]. Multiple comparisons using post hoc Tukey HSD analysis showed statistically highly significant increase (P < 0.01) in mean surface roughness for both the surface-treated groups (Group II and Group III) as compared to that of the untreated group (Group I).
|Table 1: Comparative evaluation of mean surface roughness (Sa in nm) between Groups I, II, and III for overall significance by one-way analysis of variance|
Click here to view
Contact angle goniometry
The contact angle measurements and their statistical analyses for different groups (untreated and treated) were analyzed.
Contact angles ranged between 93.60° and 100.66° in Group I; 82.76° and 91.30° in Group II; and 66.12° and 69.48° in Group III. The mean contact angles of Group I, Group II, and Group III test samples were 98.26°, 86.77°, and 68.03°, respectively.
One-way ANOVA revealed overall significant differences between the mean contact angles of the three test groups, indicating highly significant differences between their wettabilities (hydrophilicity). Multiple comparisons using post hoc Tukey HSD analysis showed statistically and highly significant decrease in mean contact angles for both the surface-treated groups (Group II and Group III) as compared to that of untreated group (Group I), indicating increased wettability for both the surface-treated groups (P < 0.01; highly significant).
X-ray diffractograms obtained for all the three test groups (untreated, sandblasted, and UVP samples) before surface treatments, revealed the presence of predominantly tetragonal zirconia peaks, with negligible presence of monoclinic phase, indicating no crystal phase transformation occurring due to any of the surface treatments. The peak of the tetragonal phase was identified at 30° for all three test groups.
After immersion in SBF for 21 days, X-ray diffractograms obtained for all the three test groups (untreated, sandblasted, and UVP samples) revealed the presence of predominantly tetragonal zirconia peaks, with negligible presence of monoclinic phase, indicating no crystal phase transformation occurring due to any of the surface treatments. The peak of the tetragonal phase was identified at 30° for all three test groups.
Energy dispersive X-ray spectroscopy
Pre-immersion EDX spectrums of untreated and respective surface-treated samples revealed the presence of the elements, zirconium (Zr) (32.66%–40.26%), oxygen (O2) (53.59%–61.31%), yttrium (Y) (5.28%–5.71%), aluminum (Al) (0.13%–0.38%), and hafnium (Hf) (0.16%–0.19%) in all test groups.
Post-immersion EDX results revealed a higher Ca/P ratio for both the surface-treated groups as compared to the untreated group [Table 2]. Group III (UVP) showed the highest Ca/P ratio of 2.08, followed by Group II (sandblasted) and Group I (untreated) with ratios of 1.79 and 1.15, respectively.
|Table 2: Microchemical surface elemental composition of zirconia disc samples in Group I (untreated), Group II (sandblasted), and Group III (UV irradiated) before and after immersion in simulated body fluid|
Click here to view
The results of bioactivity tests are represented in [Table 3].
|Table 3: Comparative evaluation of the difference between the pre-immersion calcium content (reference value) and the mean post-immersion calcium content obtained for Groups I, II, and III, using Student’s paired t test|
Click here to view
Ca-SBF analysis to detect calcium content in SBF before the immersion of test samples revealed calcium content to be in the range of 153–163 mg/L with a mean of 159 mg/L. This mean value was considered as the pre-immersion reference value and used for comparing with the post-immersion calcium content in SBF obtained for the three test groups.
Student’s paired t test revealed significant reduction (P < 0.05) in the mean post-immersion calcium content of Groups I, II, and III as compared to the pre-immersion calcium content (reference value), indicating significant bioactivity for untreated as well as both the surface-treated groups [Table 3]. One-way ANOVA revealed overall significant difference between the mean post-immersion calcium content values of three test groups (P < 0.01). Multiple comparisons using post hoc Tukey HSD analysis showed statistically significant and greater decrease (P < 0.05) in the mean post-immersion calcium contents in SBF for both the surface-treated groups (Groups II and III) as compared to that of the untreated group (Group I).
| Discussion|| |
Zirconia ceramics became a prevalent biomaterial in restorative dentistry, and subsequently, research for using it as a nonmetallic implant material is on the rise.,,,
Sandblasting (airborne particle abrasion) is a subtractive method and is used to improve the surface area available for bonding. The major advantage of sandblasting is that it not only cleans organic contaminants from zirconia surface but also modifies its energy, wettability, and microroughness, thus increasing bonding area and promoting osseointegration., However, studies exploring sandblasting as a surface treatment method have yielded mixed results. The main shortcoming with sandblasting is the appearance of flaws, pits, and microcracks on the surface that can induce t–m phase changes.,, These shortcomings can be overcome by using low blasting pressure, low particle size, and short blasting distances., Controlled sandblasting technique results in microroughened surface that has been suggested to improve the osseointegration potential. Most studies have tested bioactivity of zirconia by coupling sandblasting with acid etching.,,
Studies focusing on the effect of sandblasting alone on improving the bioactivity of zirconia are sparse,,,, and hence merit further investigation. Sandblasting was done using 50-µm-sized alumina, as particle sizes >100 µm are reported to cause reduction in flexural strength, induce t–m phase transformation, and material loss. The blasting procedure was carried out as per recommendations in the literature.,,,,,
Recently, researchers have turned their focus on the development of UVP for surface modification of zirconia as it is a simple and inexpensive surface treatment modality to enhance the osseointegration potential of zirconia without compromising its structural changes.,,,, It not only imparts changes in the surface roughness and topography, but also makes the zirconia surface “superhydrophilic” by reducing the hydrocarbon contamination of surfaces to very low levels, which are prime factors for bioactivity and enhanced osseointegration.,,,, UVP can be accomplished by ultraviolet A (UVA) and ultraviolet C (UVC) types of irradiation. In this study, UVC irradiation was selected as it has been reported to enhance the bioactivity by altering the biological properties without compromising the physical characteristics and mechanical properties. UVC irradiation works through photolytic degradation mechanism/concept unlike the photocatalytic degradation in UVA treatment. The UVP protocols in this study were as per those stated in literature.,
Kokubo and Takadama had reported that in vivo apatite formation could be replicated appreciably under in vitro conditions by immersing samples in SBF at physiologic temperature (37°C). In such studies, the calcium content of freshly prepared SBF before immersion of test samples and the post-immersion calcium content in SBF are assessed. The extent of calcium depletion in SBF is indicative of the test sample’s bioactive potential. This method is also referred to as the “biomimetic” method. The bioactivity findings are also corroborated by surface characteristics analysis of test samples to assess apatite formation, topography, composition, and so on, for correlation of test results.,
Thus, the assessment of crystal phase after surface treatments assumes importance and significance because any phase change implies that the material is more prone to surface degradation. XRD analysis was used to assess the types of crystalline phase on the zirconia surfaces as recommended in the literature.,,
Surface texture or roughness is an important parameter affecting osseointegration. In vitro studies have shown increased osteoblast proliferation and apatite formation on roughened surfaces. Surface roughness at a micrometer resolution has been studied in previous studies for various implant biomaterials., However, the implant surface 3D topography at a nanolevel has been suggested to be important in determining the extent of bioactivity, and to eliminate implant rejection.,,,, The effect of surface treatments of zirconia on its bioactivity was evaluated in this study, by using the SBF method, due to previously mentioned advantages of testing bioactivity in SBF, by following the recommended protocols.,,,, Considering the impact of the composition and preparation of SBF on test outcomes, the guidelines for the same as recommended in the literature were strictly adhered to. In the preparation of SBF, it has been reported that apatite nucleation can be induced at the surface of a glass container or the edge of scratches in such containers, which could mask the actual test results. Hence, new plastic containers and polypropylene test tubes with smooth surfaces were used for preparation and immersion of the test samples in SBF. All test samples were individually immersed in test tubes containing equal volumes of SBF, and incubated at 37°C for 3 weeks to ensure standardized study conditions.
In this study, calcium content in SBF was assessed by ICP-MS, as this equipment has an accuracy to detect and automatically compute the percentage of any given ion concentration in a known quantity of a solution, from a 1-mL sample dose. Multiple measurements of the calcium ion concentration in SBF were randomly done in this study to ascertain the standardization of SBF preparation procedure, and the mean pre-immersion Ca content was obtained and kept as the reference value for comparison with the post-immersion calcium content.
Different studies have tested bioactivity of zirconia in SBF by using various periods of immersion ranging from 2 days to several weeks.,,,, In a related pilot study, a 2-week immersion protocol was initially tested. However, no appreciable calcium depletion in SBF or formation of apatite on the test samples of all test groups was observed at the end of this period. This could perhaps be caused due to inherent bio-inert nature of zirconia. Hence, in this study, the immersion of test samples was done for a period of 3 weeks, which has also been considered as the minimum immersion time in previous studies.,,, The respective mean post-immersion calcium content in SBF was derived and compared with the pre-immersion calcium value to arrive at each group’s bioactivity potential. The respective mean post-immersion calcium contents in SBF of the test groups were compared to determine if there were any significant differences in their bioactivity with respect to each other. In addition, the surface characteristics of post-immersion test samples of each group were assessed to see the impact of immersion aging and apatite formation on the zirconia surfaces.
Pre-immersion XRD revealed strong tetragonal (t) peaks of zirconia with negligible “m” phase in representative diffractograms of all test groups. The highest peak of ZrO2 appeared at 30° (2θ-theta value), indicating no phase transformation due to either sandblasting or UVP. These results are suggestive of maintenance of the mechanically superior “t” zirconia crystal phase following either surface treatments and are in agreement with that observed in previous reports.,,,, The crystal phase was rechecked using XRD to determine whether immersion aging had resulted in any t–m transformation.
Surface roughness evaluation on a nanoscale by AFM of representative samples of all test groups revealed average surface roughness of 41.83 nm for Group I (untreated), 115.65 nm for Group II (sandblasted), and 102.43 nm for Group III (UVP) [Table 1]. Both types of surface treatments resulted in significantly higher surface roughness as compared to the untreated sample (P < 0.05). (Although the surface roughness achieved by sandblasting was slightly higher than that achieved by UVP, this difference was found to be statistically insignificant [P > 0.05]. These results were correlated with the respective 2D and 3D images). These findings indicate that both types of surface treatments improve the surface roughness similarly.,,,, Previous stand-alone studies using sandblasting or UVP have reported significant increase in surface roughness as compared to untreated surfaces, and the results obtained in this study are in line with these findings.,,,,,,, Comparative studies on surface roughness caused by these surface treatments are lacking, and hence further correlations on this aspect of this study results cannot be drawn.
The mean contact angles of 98.26° for Group I (untreated) samples, 86.77° for Group II (sandblasted) samples, and 68.03° for Groups III (UVP) samples, respectively, were obtained in this study. On comparison, these differences were found to be highly significant (P value <0.01) between all the three test groups. These results indicate that both types of surface treatments significantly improve the surface wettability as compared to that of the untreated surface, and are in accordance with those reported in the literature.,,,,,, The mechanism of improving the wettability due to surface treatments by lowering the surface energy has been previously mentioned. Surface wettability of 90° and more have been categorized as hydrophobic and “superhydrophobic.” Contact angles <90° have been said to be hydrophilic and those that are between 0° and 30° are said to be highly hydrophilic or superhydrophilic. It has been reported that UVP treatment renders the surface superhydrophilic. In such studies, a wide range of contact angles after UVP treatment, ranging from 0° to 34°, has been reported.,,,,,, The literature reported range is considerably lower than the wettability angles observed after UVP treatment in this study, and hence this study values can be termed as hydrophilic behavior, instead of superhydrophilic. This could be attributed to differences in study environment, and the number of samples tested in this study and merits further investigation. However, the fact that UVP significantly improved the hydrophilicity as compared to untreated and sandblasted surfaces is established here, which is in line with that stated in previous studies.,,,,
In this study, SEM photomicrographs revealed significant variations in the microtopographies of the untreated and treated samples. Group I (untreated) sample showed flattened areas with isolated patches of moderately roughened surface, whereas Group II (sandblasted) sample showed nonuniform, irregular surface with accentuated peaks and valleys and Group III (UVP) sample showed uniformly roughened surface with a coral-like appearance. Both the surface-treated groups were marked by the absence of flattened areas as observed with the untreated sample. These observations indicated that surface topography is altered due to both types of surface treatments and UVP, resulting in a more uniformly textured surface, which are in line with those observed in previous studies.,,,,, Respective EDX spectrums revealed the presence of the elements, Zr (32.66%–40.26%), O2 (53.59%–61.31%), Y (5.28%–5.71%), Al (0.13%–0.38%), and Hf (0.16%–0.19%) in all test groups [Table 2], indicating that both surface treatments do not alter the elemental composition of zirconia, as compared to the untreated sample. Thus, the stabilizing elements added by the manufacturer have been retained even after the surface treatment procedures. These findings are in line with those reported in previous EDX spectrum reports.,,
The mean pre-immersion Ca content of SBF was found to be 159 mg/L, and this was used as the reference value for calculating bioactivity. Previous bioactivity studies using SBF have reported a mean calcium content of 100–160 mg/L in freshly prepared SBF.,, The reference value obtained in this study was within the literature reported range. Group I (untreated) showed a mean post-immersion Ca content of 70.10 mg/L, Group II (sandblasted) showed a mean post-immersion Ca content of 60.80 mg/L, and Group III (UVP) showed a mean post-immersion Ca content of 56.20 mg/L at the end of 3 weeks. The difference between the mean pre- and post-immersion Ca contents in SBF, which is observed is due to the precipitation of calcium-rich apatite phase on the zirconia test surfaces. The lower the post-immersion Ca content in SBF, the higher the bioactivity for that particular test group.
On comparison, the respective mean post-immersion Ca content in SBF for all the three test groups showed statistically significant calcium depletion when compared with the pre-immersion Ca content, indicating highly significant bioactivity for untreated as well as both the surface test groups [Table 3] (P value <0.01). Although the results of this study indicate significant bioactive potential for untreated zirconia by virtue of the calcium depletion observed after immersion, this bioactivity was found to be significantly lesser as compared to that of both the surface-treated groups (P value <0.01). On comparison between the two types of surface treatments, Group II (sandblasted) had lesser post-immersion calcium depletion in SBF than that of Group III (UVP). However, this difference was found to be statistically insignificant (P value >0.01), indicating similar bioactive potential for both the types of surface treatments used in this study.
Thus, despite being categorized as a bio-inert ceramic, there is a definite apatite-forming tendency on untreated zirconia at the end of a 3-week immersion period.,, However, this apatite layer formed on the untreated sample was found to be a poorly defined, discontinuous layer of bone-like apatite in the form of scattered crystals, with the evidence of uncovered zirconia substrate at certain locations, as evidenced in the post-immersion SEM image. The post-immersion SEM images for Groups II and III also corroborate this finding of superior bioactivity, in that their apatite layer was made of denser, larger crystals with a continuous surface topography, and the zirconia substrate was not visible in any of the observed fields. Group II (sandblasted) sample showed dense, large, irregular, crystal-like calcium apatite deposits of irregular density and distribution, and Group III (UVP) showed profuse, rectangular crystal-like calcium apatite deposits with greater uniformity in size, density, and distribution. Moreover, the post-immersion EDX results revealed a higher Ca/P ratio for both the surface-treated groups as compared to the untreated group [Table 2]. Group III (UVP) showed the highest Ca/P ratio of 2.08, followed by Group II (sandblasted) and Group I (untreated) with ratios of 1.79 and 1.15, respectively. It has been reported in the literature that Ca/P ratio of 1.50 indicates apatite formation similar to trabecular bone, whereas values upward of 1.60 indicate cortical bone-like apatite formation. When viewed in this perspective, the low Ca/P ratio for the untreated group is indicative of trabecular bone formation, whereas both surface-treated groups in this study had Ca/P ratios indicative of cortical bone formation, indicating their superiority as surface treatment methods. All these findings suggest that surface treatment of zirconia serves to significantly enhance its bioactive potential and also results in apatite layer of superior quality as compared to an untreated surface. These findings are echoed in the results of previous studies that have evaluated the bioactivity of zirconia following surface treatment by either sandblasting or UVP.,,, These results are also in agreement with literature quoting that untreated zirconia surfaces also attract the calcium present in SBF, but to a diminished extent, as compared to any type of surface treatment. Surface treatments are said to promote bioactivity, as they remove impurities, reduce surface hydrocarbons, increase surface energy, thereby providing improved surface characteristics such as roughness and wettability, which are critical in promoting cell adhesion and calcium apatite formation. Previous studies evaluating the efficacy of sandblasting and UVP surface treatments have reported improved cell adhesion and osseointegration. The results of superior bioactivity in SBF obtained after these two types of surface treatments in this study complement the results obtained from previous cell culture studies.,,,,,
Further, post-immersion X-ray diffractograms revealed that there was no detectable crystalline phase change from tetragonal to monoclinic (t–m transformation), following a 3-week immersion. The post-immersion EDX revealed the presence of all the surface elements that were detected in the pre-immersion EDX, and additionally calcium and phosphorus were also detected attributable to apatite formation. This prevention of t–m transformation, even after immersion, can be attributed to the maintained presence of the stabilizing elements such as yttrium and hafnium that were added by the manufacturer. Thus, even after a 3-week immersion or aging, there was no detectable low temperature degradation (LTD), which is usually the area of concern with using zirconia ceramic as an implant biomaterial. The results obtained with this study serve as an encouragement for use of zirconia as an implant biomaterial, with respect to this finding.
In this study, both sandblasting and UVP surface treatments resulted in insignificant differences with respect to their bioactivity in SBF, and hence the null hypothesis of this study is validated. Therefore, it can reasonably be assumed within the limitations of this study that either of these surface treatments can be used to significantly and probably, similarly, improve the bioactivity of zirconia. This is especially so, given that both these types of surface treatments significantly improved surface roughness and wettability, without deleteriously affecting their surface crystalline phase (“t” phase) and their elemental composition. Although in this study, superior surface wettability was observed after UVP as compared to sandblasting, the bioactivity of the UVP samples was only marginally and insignificantly higher in comparison. Wettability has been repeatedly emphasized as one of the key determinants in deciding the bioactive potential of a material, both with respect to the apatite-forming ability as well as for improving cellular adhesion.,,, Hence, the apparent lack of a significantly superior bioactive potential after UVP surface treatment, over that obtained after sandblasting surface treatment needs to be considered, especially given the fact that UVP-treated samples showed significantly superior wettability as compared to the sandblasted samples. This can be attributed to the lesser number of samples that were investigated in this study and merits further investigation. Moreover, bioactivity studies in SBF comparing these type of surface treatments are lacking in the literature, and hence further correlations with the results obtained in this study cannot be drawn to arrive at better conclusions. Though it is well accepted that chemical and topographical aspects of surface texture are important in playing a vital role in osseointegration,, the exact effect of this aspect on bioactivity is still the object of investigation by researchers.
| Conclusion|| |
Both surface treatments, sandblasting with 50 µm alumina and UVP irradiation improved the bioactivity as compared to the untreated group, and this is supported by results of surface characteristic analyses. Both surface treatments did not alter the structure and mechanical property of zirconia.
Enhanced bioactivity due to surface treatments in vitro signifies enhanced osseointegration potential of surface-treated implants in vivo.
We sincerely thank Dr. Saminaidu Krishnaveni, PhD, former professor and head, Department of Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India, for her valuable inputs.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Abd El-Ghany OS, Sherief AH Zirconia based ceramics, some clinical and biological aspects: review. Future Dent J 2016;2:55-64.
Osman RB, Swain MV A critical review of dental implant materials with an emphasis on titanium versus zirconia. Materials (Basel) 2015;8:932-58.
Saini M, Singh Y, Arora P, Arora V, Jain K Implant biomaterials: a comprehensive review. World J Clin Cases 2015;3:52-7.
Tuna T, Wein M, Altmann B, Steinberg T, Fischer J, Att W Effect of ultraviolet photofunctionalisation on the cell attractiveness of zirconia implant materials. Eur Cell Mater 2015;29:82-94; discussion 95-6.
Best SM, Porter AE, Thian ES, Huang J Bioceramics: past, present and for the future. J Eur Ceram Soc 2008;28:1319-30.
Dehestani M, Ilver L, Adolfsson E Enhancing the bioactivity of zirconia and zirconia composites by surface modification. J Biomed Mater Res B Appl Biomater 2012;100:832-40.
Ke J, He F, Ye J Enhancing the bioactivity of yttria-stabilized tetragonal zirconia ceramics via grain-boundary activation. ACS Appl Mater Interfaces 2017;9:16015-25.
Rocchietta I, Fontana F, Addis A, Schupbach P, Simion M Surface-modified zirconia implants: tissue response in rabbits. Clin Oral Implants Res 2009;20:844-50.
Ewais OH, Al Abbassy F, Ghoneim MM, Aboushelib MN Novel zirconia surface treatments for enhanced osseointegration: laboratory characterization. Int J Dent 2014;2014:203940.
Liu X, Huang A, Ding C, Chu PK Bioactivity and cytocompatibility of zirconia (ZrO(2)) films fabricated by cathodic arc deposition. Biomaterials 2006;27:3904-11.
Nguyen Thi TP, Jeong OG, Pil LH, Dug YK, Won KJ, Thi VV, et al
. Evaluation of sandblasting on mechanical properties and cell response of bioactive glass infiltrated zirconia. J Nanosci Nanotechnol 2017;17:2740-2.
Roy M, Pompella A, Kubacki J, Piosik A, Psiuk B, Klimontko J, et al
. Photofunctionalization of dental zirconia oxide: surface modification to improve bio-integration preserving crystal stability. Colloids Surf B Biointerfaces 2017;156:194-202.
Tuna T, Wein M, Swain M, Fischer J, Att W Influence of ultraviolet photofunctionalization on the surface characteristics of zirconia-based dental implant materials. Dent Mater 2015;31:e14-24.
Watanabe H, Saito K, Kokubun K, Sasaki H, Yoshinari M Change in surface properties of zirconia and initial attachment of osteoblast-like cells with hydrophilic treatment. Dent Mater J 2012;31:806-14.
Brezavscek M, Fawzy A, Bächle M, Tuna T, Fischer J, Att W The effect of UV treatment on the osteoconductive capacity of zirconia-based materials. Materials 2016;9:958-77.
Casucci A, Osorio E, Osorio R, Monticelli F, Toledano M, Mazzitelli C, et al
. Influence of different surface treatments on surface zirconia frameworks. J Dent 2009;37:891-7.
Hallmann L, Ulmer P, Reusser E, Hämmerle C Effect of blasting pressure, abrasive particle size and grade on phase transformation and morphological change of dental zirconia surface. Surface Coatings Technol 2012;206:4293-302.
Strickstrock M, Rothe H, Grohmann S, Hildebrand G, Zylla I, Liefeith K Influence of surface roughness of dental zirconia implants on their mechanical stability, cell behavior and osseointegration. BioNanoMat 2017;18:1-13.
Kirmali O, Kustarci A, Kapdan A Surface roughness and morphologic changes of zirconia: effect of different surface treatment. Niger J Clin Pract 2015;18:124-9.
Bächle M, Butz F, Hübner U, Bakalinis E, Kohal RJ Behavior of CAL72 osteoblast-like cells cultured on zirconia ceramics with different surface topographies. Clin Oral Implants Res 2007;18:53-9.
Queiroz JRC, Paulo GPM, Ozcan M, Nogueira L Jr. Effect of airborne particle abrasion protocols on surface topography of Y-TZP ceramic. Ceramica 2012;58:253-61.
Tuladhar SL, Parajuli U, Wang H Response of MC3T3-E1 cells on microroughened bioactive glass coated zirconia. JCMS Nepal 2017;13:350-6.
Kenawy MH, El-Hadad AA, Soliman IE, Ereiba KMT Bioactivity and characterization. Study of synthetic zirconia-silicate sol-gel glass powder. Middle East J Appl Sci 2016;6:329-40.
Albrektsson T, Zarb GA Current interpretations of the osseointegrated response: clinical significance. Int J Prosthodont 1993;6:95-105.
Kokubo T, Takadama H How useful is SBF in predicting in vivo
bone bioactivity? Biomaterials 2006;27:2907-15.
Uchida , Kokubo T, Kawashita M, Neo M Apatite-forming ability of zirconia/alumina composite induced by chemical treatment. J Biomed Mater Res 2002;60:277-82.
Wang G, Meng F, Ding C, Chu PK, Liu X Microstructure, bioactivity and osteoblast behavior of monoclinic zirconia coating with nanostructured surface. Acta Biomater 2010;6: 990-1000.
Wang XX, Yan W, Hayakawa S, Tsuru K, Osaka A Apatite deposition on thermally and anodically oxidized titanium surfaces in a simulated body fluid. Biomaterials 2003;24: 4631-7.
Kohal RJ, Wolkewitz M, Hinze M, Han JS, Bächle M, Butz F Biomechanical and histological behavior of zirconia implants: an experiment in the rat. Clin Oral Implants Res 2009;20: 333-9.
Kim YH, Koak JY, Chang IT, Wennerberg A, Heo SJ A histomorphometric analysis of the effects of various surface treatment methods on osseointegration. Int J Oral Maxillofac Implants 2003;18:349-56.
Schliephake H, Hefti T, Schlottig F, Gédet P, Staedt H Mechanical anchorage and peri-implant bone formation of surface-modified zirconia in minipigs. J Clin Periodontol 2010;37:818-28.
Altmann B, Kohal RJ, Steinberg T, Tomakidi P, Bächle-Haas M, Wennerberg A, et al
. Distinct cell functions of osteoblasts on UV-functionalized titanium- and zirconia-based implant materials are modulated by surface topography. Tissue Eng Part C Methods 2013;19:850-63.
Att W, Takeuchi M, Suzuki T, Kubo K, Anpo M, Ogawa T Enhanced osteoblast function on ultraviolet light-treated zirconia. Biomaterials 2009;30:1273-80.
Han JM, Hong G, Matsui H, Shimizu Y, Zheng G, Lin H, et al
. The surface characterization and bioactivity of NANOZR in vitro
. Dent Mater J 2014;33:210-9.
Karamian E, Khandan A, Motamedi MR, Mirmohammadi H Surface characteristics and bioactivity of a novel natural HA/zircon nanocomposite coated on dental implants. Biomed Res Int 2014;2014:410627.
Yoshida E, Hayakawa T Quantitative analysis of apatite formation on titanium and zirconia in a simulated body fluid solution using the quartz crystal microbalance method. Adv Mat Sci Eng 2017;928379:1-9.
Gupta S A recent updates on zirconia implants: a literature review. Dent Implants Dentures 2016;1:113.
Hisbergues M, Vendeville S, Vendeville P Zirconia—established facts and perspectives for a biomaterial in dental implantology. J Biomed Mater Res B Appl Biomater 2009;88:519-29.
Madfa AA, Al-Sanabani FA, Al-Qudami NH, Al-Sanabani JS, Amran AG Use of zirconia in dentistry: an overview. Open Biomat J 2014;5:1-9.
Depprich R, Zipprich H, Ommerborn M, Naujoks C, Wiesmann HP, Kiattavorncharoen S, et al
. Osseointegration of zirconia implants compared with titanium: an in vivo
study. Head Face Med 2008;11:40-55.
Ponche A, Bigerelle M, Anselme K Relative influence of surface topography and surface chemistry on cell response to bone implant materials. Part II: biological effects. Proc Inst Mech Eng 2010;224:1487-507.
Zhang Y, Lawn BR, Rekow ED, Thompson VP Effect of sandblasting on the long term performance of dental ceramics. J Biomed Mater Res B Appl Biomater 2004;71:381-6.
Zinelis S, Thomas A, Syres K, Silikas N, Eliades G Surface characterization of zirconia dental implants. Dent Mater 2010;26:295-305.
Ferguson SJ, Langhoff JD, Voelter K, von Rechenberg B, Scharnweber D, Bierbaum S, et al
. Biomechanical comparison of different surface modifications for dental implants. Int J Oral Maxillofac Implants 2008;23:1037-46.
Noro A, Kaneko M, Murata I, Yoshinari M Influence of surface topography and surface physicochemistry on wettability of zirconia (tetragonal zirconia polycrystal). J Biomed Mater Res B Appl Biomater 2013;101:355-63.
Gahlert M, Gudehus T, Eichhorn S, Steinhauser E, Kniha H, Erhardt W Biomechanical and histomorphometric comparison between zirconia implants with varying surface textures and a titanium implant in the maxilla of miniature pigs. Clin Oral Implants Res 2007;18:662-8.
Borges GA, Sophr AM, de Goes MF, Sobrinho LC, Chan DC Effect of etching and airborne particle abrasion on the microstructure of different dental ceramics. J Prosthet Dent 2003;89:479-88.
Della Bona A, Donassollo TA, Demarco FF, Barrett AA, Mecholsky JJ Jr. Characterization and surface treatment effects on topography of a glass-infiltrated alumina/zirconia-reinforced ceramic. Dent Mater 2007;23:769-75.
Paes PNG, Bastian FL, Jardim PM The influence of Y-TZP surface treatment on topography and ceramic/resin cement interfacial fracture toughness. Dent Mater 2017;33:976-89.
Yang Y, Zhou J, Liu X, Zheng M, Yang J, Tan J Ultraviolet light-treated zirconia with different roughness affects function of human gingival fibroblasts in vitro
: the potential surface modification developed from implant to abutment. J Biomed Mater Res B Appl Biomater 2015;103:116-24.
[Table 1], [Table 2], [Table 3]