Journal of Pharmacy And Bioallied Sciences

ORIGINAL/BRIEF
Year
: 2012  |  Volume : 4  |  Issue : 5  |  Page : 31--32

Method optimization of ocular patches


Kamalesh Upreti, Prashant Dabral, Vinod Rana, Kumud Upadhyaya, Anjali Badhani 
 Department of Pharmacy, Gyani Inder Singh Institute of Professional Studies, Dehradun, Uttarakhand, India

Correspondence Address:
Kamalesh Upreti
Department of Pharmacy, Gyani Inder Singh Institute of Professional Studies, Dehradun, Uttarakhand
India

Abstract

The intraocular patches were prepared using gelatin as the polymer. Ocular patch were prepared by solvent casting method. The patches were prepared for six formulations GP1, GP2, GP3, GP4, GP5 and GP6. Petri dishes were used for formulation of ocular patch. Gelatin was used as a polymer of choice. Glutaraldehyde used as cross linking agent and (DMSO) dimethylsulfoxide used as solubility enhancer. The elasticity depends upon the concentration of gelatin. 400 mg amount of polymer i.e gelatin gave the required elasticity for the formulation.



How to cite this article:
Upreti K, Dabral P, Rana V, Upadhyaya K, Badhani A. Method optimization of ocular patches.J Pharm Bioall Sci 2012;4:31-32


How to cite this URL:
Upreti K, Dabral P, Rana V, Upadhyaya K, Badhani A. Method optimization of ocular patches. J Pharm Bioall Sci [serial online] 2012 [cited 2019 Oct 23 ];4:31-32
Available from: http://www.jpbsonline.org/text.asp?2012/4/5/31/94130


Full Text

Conventional dosage forms are easy to instill but suffer from the inherent drawback that the majority of the medication they contain is immediately diluted in the tear film as soon as the eye drop solution is instilled into the cul-de-sac and is rapidly drained away from the precorneal cavity by constant tear flow and lacrimo-nasal drainage. Therefore only a very small fraction of the instilled dose is absorbed by the target tissue for this reason, concentrated solutions and frequent dosing are required for the instillation to achieve an adequate level of therapeutic effect. Different methods were used for the formulation of ocular patch. Initially a mould was prepared with the help of glass plate and metallic strips. But the final formulation was not easily peeled off from the mould, not uniform in thickness and not very clear. Considering these problem, finally petri-dishes were selected as mould for the formulation of ocular patch. Ocular patches made by this technique were easily peeled off and are clear too.

 Materials and Methods



Formulation design and method of preparation of drug loaded ocular patch: Ocular patch were prepared by solvent casting method. Petri dishes were used for formulation of ocular patch. Gelatin was used as a polymer of choice. Glutaraldehyde used as cross linking agent [1] and (DMSO) dimethylsulfoxide used as solubility enhancer.

Required quantity of polymer (400 mg) was weighed accurately and was transferred to beaker. 5 ml of distilled water was added and mixed thoroughly with the help of magnetic stirrer and the formulation without drug was autoclaved. Autoclave formulation was taken to a sterilized room and the sealed beaker was opened under laminar air hood. Dimethyl sulfoxide (400 μl) was added with continuous stirring. Different concentration of drug (Natamycin) was added to the solution and mixed thoroughly. Then at last when drug was properly mixed then GTA (Glutaraldehyde, Cross-linking agent) was added 100 μl (10%). With this the formulation was immediately poured into Petri dish to form patch of uniform thickness. [2] Then the patch was allowed to dry under laminar air for 4 hours. Then the patch was peeled using blade, cut into 3 mm × 3 mm, transferred to poly pack, sealed and stored at room temperature till further studies.

 Results and Discussion



The intraocular patches were prepared using gelatin as the polymer of choice to formulate different formulation. The patches were prepared for six formulations GP1, GP2, GP3, GP4, GP5 and GP6. During the formulation the trouble was concentration of polymer. The elasticity of the patch was the primary evaluating factor. The elasticity depends upon the concentration of gelatin. The formulation was tried with different concentration. Finally 400 mg amount of polymer i.e gelatin gave the required elasticity for the formulation [Table 1].{Table 1}

 Conclusion



Conventional dosage forms are easy to instill but suffer from the inherent drawback that the majority of the medication they contain is immediately diluted in the tear film as soon as the eye drop solution is instilled into the cul-de-sac and is rapidly drained away from the precorneal cavity by constant tear flow and lacrimo-nasal drainage. Therefore only a very small fraction of the instilled dose is absorbed by the target tissue for this reason, concentrated solutions and frequent dosing are required for the instillation to achieve an adequate level of therapeutic effect. To overcome this problem, various drug delivery devices have been used and intracorneal patches are one of them.

References

1Rathore KS, Neema RK. An Insight into Ophthalmic drug delivery. Indian J Pharm Sci Drug Res 2009;1:1-5.
2O'Day DM, Ray WA, Robinson RD, Head WS, Savage AM. in vitro and in vivo susceptibility of candida keratitis to topical polyenes. Invest Ophthalmol Vis Sci 1987;28:874-80.