Journal of Pharmacy And Bioallied Sciences

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 11  |  Issue : 1  |  Page : 16--22

High-performance liquid chromatographic analysis explores the potential antioxidative agents of Argyreia argentea ARN. EX CHOISY extract


Md. Nazim Uddin1, Md. Atiar Rahman2, Hemayet Hossain1, Tanzir Ahmed Khan1, Rehana Akter1 
1 Institute of Food Science and Technology, Bangladesh Council of Scientific and Industrial Research, Dhaka 1205, Bangladesh
2 Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong 4331, Bangladesh

Correspondence Address:
Dr. Md. Atiar Rahman
Associate Professor, Department of Biochemistry & Molecular Biology, University of Chittagong, Chittagong-4331
Bangladesh

Background: Antioxidative properties of medicinal plants play the key role in plant defense mechanism. Argyreia argentea is an evergreen shrub which is used in the treatment of boils, gastric ulcers, tumor, marasmus, paralysis and spermatorrhea, rheumatoid arthritis, cold, painful sensation, and fever. Aims: This research investigates the phytochemical contents and antioxidative effects of optimized crude methanol extract of A. argentea. Materials and Methods: Crude methanol extract of A. argentea prepared in an optimized procedure has been analyzed by high-performance liquid chromatography to quantitatively determine the phytochemical contents. Tannin content of the extract was determined by established method. The extract was also analyzed for in vitro antioxidative actions by spectrophotometric analysis using 2,2’-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS) method, N, N-dimethyl-1,4-diaminobenzene (DMPD) free radical scavenging method, superoxide radical scavenging method, and nitric oxide scavenging method. Results: The experimental results showed a high amount of catechin hydrate (348.62mg/100g of dry extract) and moderate amount of gallic acid, p-coumaric acid, and rutin hydrate in the methanol extract of A. argentea. Tannin content was found to be 29.66mg/g tannic acid equivalent. Scavenging effects expressed as inhibition concentrations (IC50) for ABTS assay, DMPD assay, superoxide assay, and NO assay were 1148.3 µg/mL ± 7.32 µmol ascorbic acid/g, 1017.68 µg/mL, 1116.89 µg/mL, 1835.23 µg/mL, respectively. All the values were compared with their respective standards. No β-carotene was detected in the extract. Conclusions: Use of A. argentea extract as a source of functional food as well as an antioxidative agent could be considered with further confirmation.


How to cite this article:
Uddin MN, Rahman MA, Hossain H, Khan TA, Akter R. High-performance liquid chromatographic analysis explores the potential antioxidative agents of Argyreia argentea ARN. EX CHOISY extract.J Pharm Bioall Sci 2019;11:16-22


How to cite this URL:
Uddin MN, Rahman MA, Hossain H, Khan TA, Akter R. High-performance liquid chromatographic analysis explores the potential antioxidative agents of Argyreia argentea ARN. EX CHOISY extract. J Pharm Bioall Sci [serial online] 2019 [cited 2019 Mar 22 ];11:16-22
Available from: http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2019;volume=11;issue=1;spage=16;epage=22;aulast=Uddin;type=0