Journal of Pharmacy And Bioallied Sciences

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 11  |  Issue : 3  |  Page : 240--247

Effect of Litsea lancifolia leaf extract on glucose transporter 4 translocation and glucose uptake in 3T3L1 cell line


Murad Alsawalha1, Abeer Mohammed Al-Subaie2, Reem Yousuf Al-Jindan3, Srinivasa Rao Bolla4, Janardhana Papayya Balakrishna5, Padma Kanchi Ravi6, Shiva Shankar Reddy Gollapalli5, Vishnu Priya Veeraraghavan7, Aruthra Arumugam Pillai5, Joel Palpath Joseph5, Surapaneni Krishna Mohan8 
1 Department of Chemical and Process Engineering Technology, Jubail Industrial College (JIC), P.O.Box 10099, Jubail Industrial City 31961, Kingdom of Saudi Arabia
2 Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, P.O.Box 2435, Dammam 31441, Kingdom of Saudi Arabia
3 Department of Microbiology, College of Medicine, Imam Abdulrahman Bin Faisal University, P.O.Box 2114, Dammam 31451, Kingdom of Saudi Arabia
4 Department of Anatomy, College of Medicine, Imam Abdulrahman Bin Faisal University, P.O.Box 2114, Dammam 31451, Kingdom of Saudi Arabia
5 Department of Biotechnology, Stellixir Biotech Private Ltd, Peenya 2nd Stage Industrial Area, Bangalore-560058, Karnataka, India
6 Department of Biotechnology, Sri Padmavati Mahila Visvavidyalayam, Padmvathi Nagar, Tirupati, Andhra Pradesh, India
7 Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, 162, P. H. Road, Velappanchavadi, Chennai – 600 077, Tamil Nadu, India
8 Department of Medical Biochemistry, College of Applied Medical Sciences in Jubail (CAMSJ), Imam Abdulrahman Bin Faisal University, P.O.Box 4030, Al Ansar Rd, Deffi, Jubail Industrial City, Al Jubail 35816, Kingdom of Saudi Arabia

Correspondence Address:
Dr. Surapaneni Krishna Mohan
Department of Medical Biochemistry, College of Applied Medical Sciences–Jubail (CAMSJ), Imam Abdulrahman Bin Faisal University, P. O. Box 4030, Al Ansar Road, Deffi, Jubail Industrial City, Al Jubail 35816
Kingdom of Saudi Arabia

Background: Numerous synthetic drugs have been recommended as a remedy for diabetes, but their role in hypoglycemic effects are diverse. The side effects associated with these drugs due to their extended use led scientists to find unconventional medicines with no or little side effects. Aim: This study was aimed at assessment of in vitro antidiabetic activities of methanolic extract of Litsea lancifolia leaves by using 3T3L1 cell line. Materials and Methods: The cytotoxic effect of the leaf extract was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The glucose uptake–inducing capabilities and its correlation with glucose transporter 4 (GLUT4) translocation were measured by flow cytometry in 3T3L1 cells. In addition, the inhibitory effect of L. lancifolia leaf extract on α-amylase activity and α-glucosidase activity was determined by colorimetric methods. Results: Different concentrations of L. lancifolia leaf extract did not show any toxicity on 3T3L1 cells, after the treatment for 24h. On stimulation with leaf extract, 60.22% and 86.26% of 3T3L1 cells showed glucose uptake and GLUT4 expression, respectively. The colorimetric assays showed that the methanolic leaf extract of L. lancifolia has a significant inhibitory effect on the activity of α-amylase enzyme and α-glucosidase enzyme with inhibitory concentration (IC50) value of 248.65 µg/mL and 229.61 µg/mL, respectively. Conclusion: On the basis of the results of this study, it is evident that L. lancifolia leaf extract showed promising anti-diabetic effect when compared to the standard drugs metformin and acarbose and was nontoxic to 3T3L1 cells. Thus, it can be further investigated to recommend as a possible alternative treatment in antidiabetic applications.


How to cite this article:
Alsawalha M, Al-Subaie AM, Al-Jindan RY, Bolla SR, Balakrishna JP, Ravi PK, Gollapalli SS, Veeraraghavan VP, Pillai AA, Joseph JP, Mohan SK. Effect of Litsea lancifolia leaf extract on glucose transporter 4 translocation and glucose uptake in 3T3L1 cell line.J Pharm Bioall Sci 2019;11:240-247


How to cite this URL:
Alsawalha M, Al-Subaie AM, Al-Jindan RY, Bolla SR, Balakrishna JP, Ravi PK, Gollapalli SS, Veeraraghavan VP, Pillai AA, Joseph JP, Mohan SK. Effect of Litsea lancifolia leaf extract on glucose transporter 4 translocation and glucose uptake in 3T3L1 cell line. J Pharm Bioall Sci [serial online] 2019 [cited 2019 Oct 21 ];11:240-247
Available from: http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2019;volume=11;issue=3;spage=240;epage=247;aulast=Alsawalha;type=0