Journal of Pharmacy And Bioallied Sciences

: 2020  |  Volume : 12  |  Issue : 5  |  Page : 20--26

Regenerative endodontics: A paradigm shift in clinical endodontics

Manoj Kumar Pulyodan1, Sunil Paramel Mohan2, Dhanya Valsan1, Namitha Divakar1, Shabna Moyin1, Shamsheer Thayyil1,  
1 Department of Conservative Dentistry and Endodontics, Sree Anjaneya Institute of Dental Sciences, Calicut, Kerala, India
2 Department of Oral Pathology and Microbiology, Sree Anjaneya Institute of Dental Sciences, Calicut, Kerala, India

Correspondence Address:
Dhanya Valsan
Department of Conservative Dentistry and Endodontics, Sree Anjaneya Institute of Dental Sciences, Calicut 673315, Kerala


Preserving the natural dentition in its normal form and function is one of the major goals of endodontic treatment. Re-establishing the lost vitality and development of root maturation in immature permanent teeth with pulp necrosis/apical periodontitis is quiet challenging clinically. The very basis of regenerative endodontics relies on the concept of tissue engineering using stem cells, biomimetic scaffold, and bioactive growth factors to regenerate the pulp tissue damaged by microbial infection, injury, or developmental defects. In clinical endodontics, this approach is referred to as a “paradigm shift.” Even though repair instead of true regeneration is achieved with current protocols, it is hoped that further research in the area of stem-cell-based tissue engineering will allow for true regeneration and improved treatment outcomes. The aim of this review is to discuss about the various aspects of regenerative endodontics, current clinical protocols, and the future of pulp regeneration techniques.

How to cite this article:
Pulyodan MK, Mohan SP, Valsan D, Divakar N, Moyin S, Thayyil S. Regenerative endodontics: A paradigm shift in clinical endodontics.J Pharm Bioall Sci 2020;12:20-26

How to cite this URL:
Pulyodan MK, Mohan SP, Valsan D, Divakar N, Moyin S, Thayyil S. Regenerative endodontics: A paradigm shift in clinical endodontics. J Pharm Bioall Sci [serial online] 2020 [cited 2020 Sep 20 ];12:20-26
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Full Text


Regenerative endodontics is one of the emerging treatment modalities in dentistry. Endodontic treatment for immature permanent teeth with pulp necrosis/apical periodontitis presents with a challenging clinical situation. The very basis of regenerative endodontics is the tissue engineering concept, which uses stem cells, scaffold, and growth factors to regenerate the pulp–dentin complex. These developments corroborate the attempts to preserve the natural roots, one of the major goals of endodontic treatment.

Apexification is the traditional treatment protocol for immature permanent teeth with pulp necrosis/apical periodontitis. Calcium hydroxide and mineral trioxide aggregate (MTA) are commonly used for apexification. The major drawbacks associated with calcium hydroxide are reduction in root strength, possibility of root fracture, and several treatment visits over a long period of time.[1],[2] In comparison with calcium hydroxide, MTA apexification requires only short treatment time. But neither of these methods encourages root maturation, which is the thickening of root canal walls and/or closure of apex. Apexification procedure offers no possibility to re-establish the vitality of the pulp tissue, damaged by microbial infection, injury, or developmental defects. Regenerative endodontic procedures (REPs) can be considered as an alternative to apexification procedure as it offers continued root development and reinforcement of dentinal walls by hard tissue deposition, thereby providing adequate strength to the root against fracture.[3]


Regenerative endodontics can be defined as “biologically based procedures designed to replace damaged tooth structures, including dentine and root structures, as well as cells of the pulp–dentine complex”.[4]


The scope of REPs in necrotic teeth was first analyzed by Nygaard-Ostby[5] in 1961. Iwaya et al.[6] in 2001 detailed about the treatment procedure termed revascularization that had the potential for root maturation. Revitalization was proposed as a more accurate term instead of revascularization as the tissues that regenerated in the root canal space were not only comprised of blood vessels but also both hard and soft tissues.[7] In 2004, Banchs and Trope proposed the protocol for revascularization of immature permanent teeth with apical periodontitis using triple antibiotic paste (TAP).[8] This was based on the findings observed from the experiments by Kling et al.,[9] Hoshino et al.,[10] and Nygard-Ostby and Hjortdal.[11]


The term “regenerative endodontics” was approved by the American Association of Endodontists (AAE) in 2007.[4] Terms such as revascularization, revitalization, and regenerative endodontics can be used interchangeably and synonymously. The term regenerative endodontic procedures cites all procedures that are directed to obtain organized repair of damaged pulp tissue and include future treatment approaches yet to unfold in regenerative endodontics.

 Difference Between Pulp Revascularization and Pulp Regeneration

Pulp revascularization is the re-establishment of vascularity or angiogenesis in the root canal.[12] It does not suggest the repopulation of odontoblasts. Pulp regeneration does not occur in the absence of revascularization and is treated incomplete if there is no odontoblastic layer lining the dentinal surface, nociceptive as well as sympathetic and parasympathetic nerve fibers, interstitial fibroblasts, and stem/progenitor cells, which replenish the pulp cells in the newly regenerated pulp tissue.

 Clinical Protocol for Regenerative Endodontic Procedure

“Clinical Considerations for Regenerative Procedure” [Table 1] proposed by AAE states that regenerative endodontic therapy (RET) is advocated for teeth with pulp necrosis and immature apex.[13] On the basis of Cvek’s[14] classification for root development [Figure 1], following recommendations were proposed:{Table 1} {Figure 1}

Immature permanent teeth with pulp necrosis at the stage 1 (less than 1/2 of root formation and open apex), stage 2 (1/2 root formation and open apex), and stage 3 (2/3 of root development and open apex) can be preferred for RET due to short root, thinned out canal walls and wide-open apex. Management of immature permanent teeth at stage 4 (nearly completed root formation and open apex) includes either RET or an apical MTA plug and a dense root canal filling because of adequate thickness and strength of the canal walls.

Immature permanent teeth with pulp necrosis requiring post and core restoration are not suitable for RET and are considered for apical MTA plug and root canal filling.

 Apical Diameter Size

The size of apical diameter is of concern as it can affect the outcome of REP in immature permanent teeth. A wide-open apex makes it easier for the mesenchymal stem cells to migrate into the root canal space, facilitating tissue ingrowth into the root canal space.[15],[16] Highest clinical success rate was seen in apical diameters of 0.5–1.0 mm.[17]

 Age of the Patient

Young patients in the age group of 9–18 years are considered as suitable candidates for REPs because of increased potential for stem cell regeneration.[18] There is risk of impairing the eruption pattern of permanent teeth when REPs are undertaken in deciduous teeth.[17]


Minimal or no filing of the canal is recommended in REPs to avoid weakening of the root canal walls.[19] The results of histologic and bacteriologic studies, however, indicate that some amount of mechanical debridement may also be required for the eradication of biofilm and for continued root maturation to occur.[19]

 Root Canal Disinfection

Effective root canal disinfection is crucial for the success of REPs because infection can interfere with stem cell activity and regeneration as well as the repair process.[19],[20] The clinical considerations for REP advocate gentle irrigation with copious amounts of an effective irrigant such as sodium hypochlorite (NaOCl). Needle with closed end and side-vents, or EndoVac™, can be used to reduce the possibility of irrigant extrusion into the periapical space.[9] To minimize the damage to stem cells in the apical region, reduced concentrations of NaOCl are recommended (1.5% NaOCl [20 mL/canal, 5 min]).[21],[22] This is followed by irrigation with saline or ethylenediaminetetraacetic acid (EDTA; 20 mL/canal, 5 min) placing the irrigation needle about 1 mm from root end. There was an increase in the survival of stem cells of the apical papilla and partial reversal of the destructive effects of NaOCl when 17% EDTA was used.[22] EDTA caused demineralization of dentine to release growth factors from the dentine matrix; improved the adhesion, migration, and differentiation of stem cells; and also enhanced the bonding of newly formed mineralized tissue to the walls of the canal.[23],[24],[25],[26]

 Intra-Canal Medicament

The use of triple antibiotic paste (TAP) or “3mix” consisting of minocycline, ciprofloxacin, and metronidazole is considered as an effective intra-canal medicament, due to its ability to eliminate all species of bacteria from the infected root canals.[9],[14] The AAE protocol recommends the use of TAPs at a concentration that is not greater than 0.1 mg/mL.[13] At this concentration, TAP did not have any toxic effect on stem cell survival and proliferation.[27] AAE guidelines also advocate the use of calcium hydroxide. The stem cells of apical papilla showed greater attachment to root dentine when treated with calcium hydroxide than with TAP.[28] Intra-canal medicament is usually placed in the canal for a period of 3–4 weeks.

 Blood Clot or Scaffold in Canal

Intentional provoking of the periapical tissues and resultant bleeding into the canal space initiate the formation of blood clot, which may act as a scaffold and provide adequate growth factors. Mesenchymal stem cells from the apical papilla are also introduced into the canal space, which will encourage regeneration of pulp tissue.[15] Platelet-rich fibrin or platelet-rich plasma can also be used as a scaffold because they are rich in growth factors that might promote the regeneration of pulp tissue.[29],[30]

 Coronal Seal

According to the current clinical protocols, a piece of CollaPlug (Zimmer Dental Inc, Warsaw, IN) must be placed over the blood clot. This serves as an internal matrix for placing of about 3 mm of white MTA (Dentsply, Tulsa, OK). A 3–4 mm layer of glass ionomer is then placed over the MTA followed by composite resin restoration over the glass ionomer.[9],[13] MTA is popular for its biocompatibility and antibacterial properties.[31] Biodentine can be used instead of MTA.[32]


According to clinical considerations for REPs (AAE[9]), success of REPs can be defined by evaluating the treatment outcomes:

Primary or essential goal is the elimination of clinical signs and symptoms and the evidence of bony healing.

Secondary or desirable goal is to achieve continued root maturation (increased thickness of root canal wall and/or increased root length)

Tertiary goal is the re-establishment of neurogenesis or positive response to vitality testing.

 Radiographic Outcomes

Chen et al.[33] described five sorts of responses for teeth treated with REPs:

Type 1—increased canal wall thickening and continued root development

Type 2—significant root development not seen (root apex is blunt and closed)

Type 3—continued root maturation but the apical foramen remains open

Type 4—presence of severe calcification or obliteration of the canal space

Type 5—formation of hard tissue barrier in the canal between the coronal MTA and the apex of the root

 Histologic Outcome—Regeneration or Repair?

Regeneration and repair are two forms of healing, after tissue injury. While former restores the normal tissue architecture and biologic functions by tissue similar to the original tissue, latter replaces the damaged tissue with tissue different from the original tissue with loss of biological functions.[34] The nature of tissue formed after RET is a controversial topic. The stem cells of apical papilla have the ability to survive even after pulp necrosis or apical periodontitis.[35] They along with the growth factors can migrate into the disinfected canal space and result in regeneration of pulp–dentin complex. However, in most of the histologic studies performed in humans and animals, the tissue found in the canal space after RET appeared to be cementum-like, periodontal ligament (PDL)-like, bone-like, and not pulp-like tissue.[36],[37] There are some studies that have reported the presence of pulp-like tissue after RET.[27],[38],[39] But in those cases, the pulp was not necrotic before the treatment and there might have been residual pulp tissue left in the canal. Therefore, based on histologic findings it can be suggested that in RET there is repair of damaged tissues and not true regeneration.

 Response to Vitality Testing

Histologic studies in human teeth have shown that the tissue found in the canal space after RET is cementum-like, bone-like, and not pulp-like tissue.[36],[37] Vital tissues being vascularized and innervated can give a positive response to pulp sensibility testing. Hence, positive response to pulp testing after RET does not always indicate regeneration of the pulp tissue.

 Limitations of Regenerative Endodontic Therapy

(1) Patient compliance can be of concern due to multiple clinical appointments and duration of the treatment.

(2) Crown discoloration associated with minocycline in TAP[40] and MTA (gray/white).[40]

(3) Histologic evidence of repair rather than true regeneration after RET.[36],[37]

(4) Root fractures were seen in some of the cases after RET.[41],[42]

(5) Cementum, PDL, and bone ingrowth into the canal space can result in internal ankylosis.[43]

(6) Intra-canal calcification has been reported after revascularization.[33]

(7) Age of the patient is crucial for the success of RET. It is likelier to be successful in younger patients than older individuals.

 Discoloration After Regenerative Endodontic Therapy

The discoloration of crown after regenerative endodontic treatment is of major concern. It is usually seen with the use of TAP consisting of minocycline.[44] There are reports of discoloration with calcium hydroxide also.[45] The pulp chamber can be sealed with dentine bonding agent to avoid the staining with TAP. The TAP should also remain below the cementoenamel junction. If there is any residual paste in the pulp chamber, it should be removed and wiped with cotton pellets soaked in absolute alcohol. Double antibiotic paste consisting of ciprofloxacin and metronidazole or modified TAP in which minocycline is substituted with clarithromycin[46] or fosfomycin[32] or cefuroxime[47] or Arestin[48] or cefaclor[49] have shown to avoid the staining. Biodentine can be used instead of MTA to reduce the risk of discoloration.[50]


Regeneration of pulp tissue is currently based on two methods: cell-free and cell-based approaches. In cell-based approach, there is isolation and ex vivo expansion of stem cells derived from host or of allogeneic nature to be seeded in the scaffold and transplanted into the root canal for regeneration.[51],[52],[53] Pulp-specific stem cells such as dental pulp stem cells,[54] stem cells of exfoliated deciduous teeth,[55] and stem cells of apical papilla are used. These stem cells result in regeneration of pulp-like tissue and formation of dentin-like tissue on walls of the canal.[49],[50],[56] However, this method has drawbacks such as difficulties in availability, isolation and expansion of stem cells, culture, handling, cost, immune rejection, government regulatory policies, and the skill of the clinician.

The cell homing approach or the cell-free approach involves the use of biologic signaling molecules for the migration, proliferation, and differentiation of endogenous stem cells.[57] Kim et al.[58] were able to regenerate tooth-like structure using cell-homing approach.[58] The signaling molecules for pulp regeneration were considered to be growth factors such as vascular endothelial growth factor, basic fibroblast growth factor, platelet-derived growth factor, nerve growth factor, and bone morphogenetic protein 7. Even though the cell homing approach is simpler, economical, and readily performed by clinicians without special training, there is lack of knowledge regarding which growth factor(s) has to be used for REPs. Current clinical REPs can be categorized as cell-free approach.


REPs in immature teeth do result in resolution of signs and symptoms of apical periodontitis and promote further root development. However, evidence suggests that true regeneration of pulp–dentin complex does not occur after RET. It is hoped that further research in the field of stem-cell-based tissue engineering will allow for true regeneration and improved treatment outcomes.

Financial support and sponsorship


Conflicts of interest

There are no conflicts of interest.


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