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  Indian J Med Microbiol
 

Figure 6b: The band (super coiled-intact DNA, open circular- DNA with single strand break and linear-DNA with double strand break) intensities were obtained using a densitometer following ethidium bromide staining and quantity of different form of plasmid DNA were estimated as described in text. The fraction of intact (super coiled) DNA calculated and plotted against corresponding treatments Figure 6a: pUC-18 plasmid was irradiated with 150 Gy γ-radiation with or without 25, 50 or 100 μg/ml of the plant extract and separated on a 0.8 % agarose gel in TAE buffer. Lane 1-control pUC-18 plasmid DNA, lane 2-pUC-18 DNA exposed to 150 Gy, lane 2-5-pUC-18 DNA treated with 25, 50 or 100 μg/ml of the plant extract for 30 min and lane 6-9- pUC DNA treated with 25, 50 or 100 μg/ml of the plant extract for 30 min before 150 Gy γ-irradiation. OC = Open circular, L = Linear and SC = Super coiled forms of the plasmid DNA,

Figure 6b: The band (super coiled-intact DNA, open circular- DNA with single strand break and linear-DNA with double strand break) intensities were obtained using a densitometer following ethidium bromide staining and quantity of different form of plasmid DNA were estimated as described in text. The fraction of intact (super coiled) DNA calculated and plotted against corresponding treatments
Figure 6a: pUC-18 plasmid was irradiated with 150 Gy γ-radiation with or without 25, 50 or 100 μg/ml of the plant extract and separated on a 0.8 % agarose gel in TAE buffer. Lane 1-control pUC-18 plasmid DNA, lane 2-pUC-18 DNA exposed to 150 Gy, lane 2-5-pUC-18 DNA treated with 25, 50 or 100 μg/ml of the plant extract for 30 min and lane 6-9- pUC DNA treated with 25, 50 or 100 μg/ml of the plant extract for 30 min before 150 Gy γ-irradiation. OC = Open circular, L = Linear and SC = Super coiled forms of the plasmid DNA,