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  Indian J Med Microbiol
 

Figure 3: DNA retention by chitosan-3-(2-aminoethyldithio) propionyl (CS-(AEDTP)) nanoparticles (NPs) after 24h of incubation with: A) Increasing concentrations of dithiothreitol; Lanes: (a) DNA marker, (b) Plasmid DNA (pDNA), (c) CS-(AEDTP) NPs, (d) to i) range from 10 mM to 100 mM according to the image. B) 0.4 M gluthatione reduced-form; Lanes: (a) DNA marker, (b) pDNA, (c) CS-(AEDTP) NPs, (d) CS-(AEDTP) NPs + NADPH + phosphate buffer, (e) CS-(AEDTP) NPs + glutathione reductase + NADPH + phosphate buffer, (f) CS-(AEDTP) NPs + PB + NADPH + phosphate buffer, (g) CS-(AEDTP) NPs + PB + GR+ NADPH + phosphate buffer. This was analyzed in a 1% agarose gel electrophoresis, with DNA visualized by GreenSafe Premium

Figure 3: DNA retention by chitosan-3-(2-aminoethyldithio) propionyl (CS-(AEDTP)) nanoparticles (NPs) after 24h of incubation with: A) Increasing concentrations of dithiothreitol; Lanes: (a) DNA marker, (b) Plasmid DNA (pDNA), (c) CS-(AEDTP) NPs, (d) to i) range from 10 mM to 100 mM according to the image. B) 0.4 M gluthatione reduced-form; Lanes: (a) DNA marker, (b) pDNA, (c) CS-(AEDTP) NPs, (d) CS-(AEDTP) NPs + NADPH + phosphate buffer, (e) CS-(AEDTP) NPs + glutathione reductase + NADPH + phosphate buffer, (f) CS-(AEDTP) NPs + PB + NADPH + phosphate buffer, (g) CS-(AEDTP) NPs + PB + GR+ NADPH + phosphate buffer. This was analyzed in a 1% agarose gel electrophoresis, with DNA visualized by GreenSafe Premium