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  Indian J Med Microbiol
 

Figure 3: PC3 and DU145 cancer cells, 24, 48, 72 and 96h after treatment with methanolic combination of juglone (×20). (A) Control; (B) 10 μg/mL after 24h; (C) 10 μg/mL after 48h; (D) 10 μg/mL after 72h; (E) 10 μg/mL after 96h; (F) 100 μg/mL after 24h; (G) 100 μg/mL after 48h; (H) 100 μg/mL after 72h; (I) 100 μg/mL after 96h; (J) 500 μg/mL after 24h; (K) 500 μg/mL after 48h; (L) 500 μg/mL after 72h; (M) 500 μg/mL after 96h; (N) 1000 μg/mL after 24h; (O) 1000 μg/mL after 48h; (P) 1000 μg/mL after 72h; and (Q) 1000 μg/mL after 96h. Arrows indicate the morphological changes in which the normal shape of cells (spindle shape) has become rounded and smaller. Also, cellular granulation was observed in them and this condition is a sign of the death of cells

Figure 3: PC3 and DU145 cancer cells, 24, 48, 72 and 96h after treatment with methanolic combination of juglone (×20). (A) Control; (B) 10 μg/mL after 24h; (C) 10 μg/mL after 48h; (D) 10 μg/mL after 72h; (E) 10 μg/mL after 96h; (F) 100 μg/mL after 24h; (G) 100 μg/mL after 48h; (H) 100 μg/mL after 72h; (I) 100 μg/mL after 96h; (J) 500 μg/mL after 24h; (K) 500 μg/mL after 48h; (L) 500 μg/mL after 72h; (M) 500 μg/mL after 96h; (N) 1000 μg/mL after 24h; (O) 1000 μg/mL after 48h; (P) 1000 μg/mL after 72h; and (Q) 1000 μg/mL after 96h. Arrows indicate the morphological changes in which the normal shape of cells (spindle shape) has become rounded and smaller. Also, cellular granulation was observed in them and this condition is a sign of the death of cells