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Year : 2010  |  Volume : 2  |  Issue : 4  |  Page : 337-340 Table of Contents     

Assessment of asthma and chronic obstructive pulmonary disorder in relation to reversibility, IgE, eosinophil, and neutrophil count in a University Teaching Hospital in South Delhi, India

Department of Pharmacology, Faculty of Pharmacy, Majeedia Hospital, Jamia Hamdard University, New Delhi - 110 062, India

Date of Submission01-May-2010
Date of Decision11-Jun-2010
Date of Acceptance03-Aug-2010
Date of Web Publication28-Oct-2010

Correspondence Address:
Razia Khanam
Department of Pharmacology, Faculty of Pharmacy, Majeedia Hospital, Jamia Hamdard University, New Delhi - 110 062
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0975-7406.72136

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Objectives : The physiological and clinical similarities between asthma and chronic obstructive pulmonary disorder (COPD) make their differentiation difficult. In the present study, we compared reversibility to bronchodilator, immunoglobulin E (IgE), blood eosinophil and neutrophil levels among asthma and COPD patients to differentiate these diseases. Materials and Methods : The study was carried on 20 asthmatics and 29 patients of COPD that reported to the outpatient and inpatient department in University Teaching Hospital, Jamia Hamdard, New Delhi, India. The parameters evaluated included pulmonary function (FEV 1 , FVC, and FEV 1 /FVC), IgE levels, and eosinophil and neutrophil count. Results : It was observed that reversibility was significantly higher in asthmatic patients, while irreversibility predominates in COPD patients. There was no significant difference in pre- and post-FEV 1 and pre- and post-FVC and in their percentage predicted. However the percentage change in FEV 1 significantly varies in asthma and COPD patients. No significant changes in neutrophil and eosinophil levels were observed in these patients. The serum IgE levels were found significantly higher in asthmatic patients. Conclusions : We conclude that reversibility in FEV 1 levels or percentage change in FEV 1 and serum IgE levels are promising lab parameter to distinguish these two conditions. However, further research is required to fully understand the role of neutrophil and eosinophil in the onset and development of asthma and COPD.

Keywords: Asthma, bronchodilator, chronic obstructive pulmonary disorder, eosinophil, immunoglobulin E, neutrophil

How to cite this article:
Rathod VP, Kapoor P, Pillai K K, Khanam R. Assessment of asthma and chronic obstructive pulmonary disorder in relation to reversibility, IgE, eosinophil, and neutrophil count in a University Teaching Hospital in South Delhi, India. J Pharm Bioall Sci 2010;2:337-40

How to cite this URL:
Rathod VP, Kapoor P, Pillai K K, Khanam R. Assessment of asthma and chronic obstructive pulmonary disorder in relation to reversibility, IgE, eosinophil, and neutrophil count in a University Teaching Hospital in South Delhi, India. J Pharm Bioall Sci [serial online] 2010 [cited 2022 Jul 3];2:337-40. Available from:

Bronchial asthma and chronic obstructive pulmonary disease (COPD) are two airway chronic diseases that exact an enormous toll on patients, healthcare providers, and the society. Asthma, where airway inflammatory component occupies a preponderant place is characterized by recurrent episodes of several symptoms such as breathlessness, wheezing, chest tightness, airway obstruction, and cough. [1] The essential alterations of asthma includes bronchospasm (hyper reactivity), edema and mucous hypersecretion (bronchial obstruction), etc. It is estimated that around 300 million people currently have asthma worldwide. [2] On the other hand, COPD is a disorder of progressive airflow limitation caused by chronic inflammation of the airways and lung parenchyma and associated with symptoms such as cough, sputum production, and dyspnoea. Smoking is the primary risk factor for the development of COPD. Worldwide, it ranks as the fourth leading cause of death, alongside HIV/AIDS. By the year 2020, COPD is predicted to become the third leading cause of death worldwide (exceeded only by heart disease and stroke). [3] Although both of them have similar characteristics such as the signs of coughing and wheezing, they are two distinct conditions in terms of disease onset, frequency of symptoms, and reversibility of airway obstruction. There has been a substantial increase in the prevalence of both diseases that has lead to sizable concerns being expressed from national and international healthcare authorities.

Exacerbations of asthma often have identifiable triggers such as allergens, cold air, or exercise. However, exacerbations in COPD patients are commonly caused by respiratory tract infections. Both these diseases were once considered to be at the opposite ends of the spectrum of airway disease, where asthma was thought to be highly responsive to treatment and essentially reversible; COPD was characterized by fixed airway narrowing and unresponsive to the treatment. The currently accepted definitions still emphasize these features, but there may be significant overlap between the two diseases. Today it is recognized that these two conditions are distinct but their clinical features overlap and it is often difficult to differentiate, clinically diagnose, and classify the two disorders. [4],[5]

Asthma and COPD are highly complex, many different inflammatory cells and multiple mediators with complex acute and chronic effects on the airways are part of the syndromes. Many different inflammatory cells are involved in asthma, although the precise role of each cell type is not yet certain. The inflammation in asthmatics airways differs strikingly from that observed in COPD, where there is a predominance of macrophages, cytotoxic (CD8+), lymphocytes, and neutrophils, although both these common diseases may coexist in some patients. Hence, the development of markers by which these two diseases can be separated out is imperative.

The criteria for diagnosis of asthma and COPD includes patients medical history, physical examination, spirometry especially pre- and post-FEV 1 , chest radiograph, differential diagnosis, etc. The present study was designed to compare asthma and COPD in relation to reversibility, IgE, eosinophils, and neutrophils count.

   Materials and Methods Top


Adult patients suffering from asthma or COPD of either sex attending the outpatient and inpatient department of University Teaching Hospital, Jamia Hamdard, New Delhi, India, were included in the study. Out of a total of 1348 patients visited, 20 patients of asthma and 29 patients of COPD were selected, on the basis of history and clinical examinations. Asthma was diagnosed by the history of recurrent episodes of breathlessness and wheezing with or without cough and phlegm with seasonal and diurnal variations and identifiable triggering factors. COPD was diagnosed on the basis of history of smoking, cough with expectoration occurring for at least 3 months in a year for 2 years or more accompanied by progressive dyspnoea on exertion.

Patients who had taken an inhaled bronchodilator within the past 12 hours or an oral bronchodilator within the past 24 hours were excluded. Patients with coronary artery disease, systemic hypertension, previous history of pulmonary tuberculosis, concurrent pulmonary disease, children, pregnant, and breast feeding females, patients on long-term antihistaminics, and patients unable to comply were also excluded. The study was carried out in accordance with the basic principles defined in 'Good Clinical Practice (GCP). It was approved by the Jamia Hamdard Institutional Review Board (JH-IRB). Informed written consent was obtained from each patient.

The characteristics of patients selected for the study are given in [Table 1].
Table 1 :Characteristics of the patients of asthma and COPD groups

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Spirometry: It was performed with subjects in sitting position and highest value of forced expiratory volume in 1 sec (FEV 1 ) and forced vital capacity (FVC) were obtained. Three acceptable and at least two reproducible curves were obtained in each subject. The baseline spirometry was then performed. Then salbutamol was administered by metered-dose inhaler. It was given by keeping it about 3 cm in front of open mouth. It was repeated 20 min after administration of salbutamol. Reversibility was calculated in asthma and COPD patient groups.

Biochemical analysis: Blood samples (5.0) were collected from each subject by venipuncture. They were analyzed for absolute eosinophil count estimation on the same day of the sampling schedule at pathology laboratory, Majeedia hospital. Absolute eosinophil count (AEC) was made from the total leukocyte count (TLC) and differential leukocyte count (DLC) by indirect method and by the direct method using light microscopy. Serum was separated and stored frozen in aliquots (0.5 ml) until analyzed for immunoglobulin E (IgE) levels. Microparticle Enzyme Immunoassay method (Calboitech, U.S.A) was used for the quantitative measurement of serum IgE.

Statistical analysis

Graphpad software was used for analyzing the data for unpaired 't' test and mean ± SEM (wherever applicable). A value of p0 <0.05 was considered significant.

   Results Top

Comparison of pulmonary functions in asthma and COPD patients

[Table 2] shows the comparison of pulmonary functions in asthma and COPD groups. Asthmatic patients showed high reversibility while irreversibility in COPD patients was higher. The reversible/irreversible ratio in asthma (15/5) is 3, whereas in COPD (4/25) is 0.16. There was no significant difference in pre- and post-FEV 1 and in also pre- and post-FVC and their percentage predicted in asthma and COPD Patients. However, percentage change in FEV 1 significantly varies in asthma and COPD patients ( p0 <0.0001, unpaired t test).
Table 2 :Comparison of pulmonary functions in asthma and COPD groups

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Comparison of blood cells in asthma and COPD patients

[Table 3] depicts the levels of blood cells in asthma and COPD group. The TLC was higher in COPD patients, though nonsignificant. Eosinophil count was slightly higher in asthma patients and the neutrophil count was higher in COPD patients, but the difference was nonsignificant.
Table 3 :Comparison of blood cells in asthma and COPD patients

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Comparison of IgE levels in asthma and COPD patients

[Table 4] illustrates the levels of IgE in asthma and COPD patients. Both the groups showed higher IgE level than the normal range, but in asthma patients it was significantly higher as compared to COPD group ( p0 <0.0001, unpaired t test).
Table 4 :Comparison of IgE levels in asthma and COPD patients

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   Discussion Top

Our study was designed with the objective whether airways reversibility, number of eosinophils, neutrophils, and IgE levels in asthma and COPD patients can be used as a marker to distinguish these diseases. It was observed that asthma patients showed high airway reversibility while COPD patients showed high airway irreversibility. These results are in agreement with other clinical studies showing the same pattern of observation. [6],[7],[8] The assessment of a bronchodilator response is a routine procedure both in pulmonary medicine and in research. These responses are commonly used as a basis for classification of disease and choice of treatment by clinicians and as an inclusion criterion for research studies. However, there is as yet no consensus as to which index best expresses bronchodilator response, or even as to what cut-off value would indicate a positive bronchodilator response. An increase in FEV 1 greater than a threshold value is assumed to represent reversibility of airways obstruction. The criteria suggested for positive test of reversibility range from 12% to 20% increase in FEV 1 , over the baseline value. [9],[10] A change in FEV 1≥9.10 of the predicted FEV 1 (European Respiratory Society) [11] and change in FEV 1≥ 12% and 0.2l over the baseline (American Thoracic Society) [12] has been specified. On the contrary, few studies have shown that these thresholds have little value in separating patients with bronchial asthma and COPD. [13],[14],[15] It is also reported that patients with COPD respond to bronchodilator more often by an increase in the FVC than in FEV 1 , hence consideration of changes in both FEV 1 and FVC to evaluate bronchodilator responsiveness has been emphasized. We have evaluated pre- and post-FEV 1 and FVC in both the patients and found that FVC increased from the baseline value after bronchodilator dosage in asthmatic as well as in COPD patients. FEV 1 also increased from the baseline after bronchodilator treatment in both the patients, but the increase was more marked in asthmatic patients. In addition, difference in percentage change in FEV 1 was statistically significant . Asthmatics showed more than 12% increase in FEV 1 after bronchodilator. On the other hand, bronchodilator response in COPD patients was limited . Thus on the basis of our observation, it was found that bronchodilator reversibility (% FEV 1 ) is a significant parameter to separate asthma from COPD.

In our study, the TLC in asthma patients was in normal range, but slightly higher in COPD patients. Deference in TLC level in both groups was also not significant. It is well-established fact that cigarette smoking is associated with an elevated leukocyte count in the peripheral blood and lungs. [16] Most of the COPD patients were smokers in our study. Slight increase in TLC in COPD patients may be due to smoking. Eosinophil count in COPD patients was in normal range, though it was higher in asthma patients but not statistically significant difference in eosinophil count was observed between these groups. Eosinophilic inflammations in asthma are well known, [17] but are also reported in the airways of some COPD patients and have a correlation with severity of airflow obstruction.

Increased neutrophil count in COPD patients was also observed, as compared to asthmatic group. It is well known that neutrophils play a crucial role in the pathophysiology of COPD, as they release multiple mediators and tissue degrading enzymes such as elastases that orchestrate tissue destruction and chronic inflammation. [18] Neutrophil numbers are also increased in bronchoalveolar lavage fluids from cigarette smokers. [19] In our study, most of the patients of COPD group were smokers. Hence its role in airways injury including COPD is a matter of speculation, and advanced as well as more targeted studies can provide a relation between neutrophils and lung injury. It has also been reported recently that COPD is associated with sputum neutrophilia and asthma with sputum eosinophilia. [20]

We have also investigated the levels of IgE with asthma and COPD patients. Since IgE molecules are known to have a role in the pathogenesis of both the diseases. In our study, we observed a significant increase in serum IgE levels of asthma patients in comparison to COPD patients. Recently, Mahajan et al. have also observed a significant increase in serum IgE levels of atopic asthmatics in comparison with nonatopic asthmatics, COPD patients, and healthy individuals. [21] Sin has shown that asthmatics also had higher serum IgE level than COPD patients, although the difference was not significant. [8] In another study, a significantly raised serum total IgE level has been detected in asthmatic patients. [7] In our study, IgE level of COPD patients was also higher than normal range, which could be due to the degree of tobacco smoking [22],[23] or local production of IgE in the bronchial mucosa. [24]

The differential diagnosis of bronchial asthma and COPD is usually made on the basis of clinical history, physical examination, and the PFT. However, in some cases, these parameters fail to establish conclusively the diagnosis of these diseases. To conclude, our study highlighted a statistically significant difference in the reversibility of FEV 1 of the magnitude of 12% in case of bronchial asthma and increased IgE levels as compared to COPD patients. Hence, it suggests that reversibility in FEV 1 level and serum IgE levels are the promising lab parameters to distinguish the conditions. Since, the study was carried out over a 4-month period, and seasonal variations in disease pattern were not considered, further studies involving more number of patients in different hospitals and the influence of inflammatory mediators on the two diseases are further warranted.

   References Top

1.Bousquet J, Jeffery PK, Busse WW, Johnson M, Vignola AM. Asthma: From bronchoconstriction to airways inflammation and remodelling. Am J Resp Crit Care Med 2000;161:1720-45.  Back to cited text no. 1
2.Masoli M, Fabian D, Holt S, Beasley R. Global Initiative for Asthma (GINA) program: The global burden of asthma: Executive summary of the GINA Dissemination Committee report. Allergy 2004;59:469-78.  Back to cited text no. 2
3.Murray CJ, Lopez AD, editors. The global burden of disease: A comprehensive assessment of mortality and disability from diseases, injuries and risk factors in 1990, and projected to 2020. Cambridge MA: Harvard University Press; 1996. p. 361-95.  Back to cited text no. 3
4.Jindal SK. Emergence of chronic obstructive pulmonary disease as an epidemic in India. Indian J Med Res 2006;124:619-30.  Back to cited text no. 4
5.Jenkins CR, Marks GB, Reddel HK. Traditional and patient-centred outcomes with three classes of asthma medication. Eur Respir J 2005;26:36-44.  Back to cited text no. 5
6.Chavannes NH, Vernooy JH, Schermer TR, Jacobs JA, Dentener MA, van Weel C, et al. Patterns of inflammation and the use of reversibility testing in smokers with airway complaints. BMC Pulm Med 2006;6:11.  Back to cited text no. 6
7.Sitkauskiene B, Sakalauskas R, Malakauskas K, Lφtvall J. Reversibility to a beta 2 agonist in COPD relationship to atopy and neutrophil activation. Respir Med 2003;97:591-8.  Back to cited text no. 7
8.Sin BA, Akkoca O, Saryal S, Oner F, Misirligil Z. Differences between asthma and COPD in the elderly. J Investig Allergol Clin Immunol 2006;16:44-50.  Back to cited text no. 8
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10.Kesten S, Rebuck AS. Is short-term response to inhaled beta adrenergic agonist sensitive or specific for distinguishing between asthma and COPD? Chest 1994;105:1042-5.  Back to cited text no. 10
11.Quanjer PH, Tammeling GJ, Cotes JE, Pedersen OF, Peslin R, Yernault JC. Lung volumes and forced ventilatory flows. Report working party standardization of lung function tests, European community for steel and coal. Official statement of the European respiratory society. Eur Respir J Suppl 1993;16:5-40.   Back to cited text no. 11
12.Lung function testing: Selection of reference values and interpretative strategies. American Thoracic Society. Am Rev Respir Dis 1991;144:1202-18.  Back to cited text no. 12
13.Meslier N, Racineux JL, Six P, Lockhart A. Diagnostic value of reversibility of chronic airway obstruction to separate asthma from chronic bronchitis: A statistical approach. Eur Resp J 1989;2:497-505.  Back to cited text no. 13
14.Brand PL, Quanjer PH, Postma DS, Kerstjens HA, Koλter GH, Dekhuijzen PN, et al. Interpretation of bronchodilator response in patients with obstructive airways disease. The Dutch Chronic Non-Specific Lung Disease (CNSLD) Study Group. Thorax 1992;47:429-36.  Back to cited text no. 14
15.Boulet LP, Turcotte H, Hudon C, Carrier G, Maltais F. Clinical physiological and radiological features of asthma with incomplete reversibility of airway obstruction compared to those of COPD. Can Respir J 1998;5:270-7.  Back to cited text no. 15
16.Schwartz J, Weiss ST. Peripheral blood leukocyte count and respiratory symptoms. Ann Epidemiol 1993;3:57-63.   Back to cited text no. 16
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18.Chung KF. Cytokines in chronic obstructive pulmonary disease. Eur Resp J 2001;34:50s-9s.  Back to cited text no. 18
19.Huninghake GW, Crystal RG. Cigarette smoking and lung destruction. Accumulation of neutrophils in the lungs cigarette smokers. Am Rev Respir Dis 1983;128:833-41.  Back to cited text no. 19
20.Caramori G, Adcock I. Pharmacology of airway inflammation in asthma and COPD. Pulm Pharmacol Ther 2003;16:247-77.  Back to cited text no. 20
21.Mahajan B, Vijayan VK, Agarwal MK, Bansal SK. Serum interleukin-1b as a marker for differentiation of asthma and chronic obstructive pulmonary disease. Biomarkers 2008;13:713-27.  Back to cited text no. 21
22.Bryon KA, Varigos GA, Wooton AM. Interleukin4 production is increased in cigarette smokers. Clin Exp Immonol 1994;95:333-6.  Back to cited text no. 22
23.Dow L, Coggon D, Campbell MJ, Osmond C, Holgate ST. The interaction between immunoglobulin E and smoking in airflow obstruction in the elderly. Am Rev Respir Dis 1992;146:402-7.  Back to cited text no. 23
24.Horwitz RJ, Busse WW. Inflammation and asthma. Clin Chest Med 1995;16:583-602.  Back to cited text no. 24


  [Table 1], [Table 2], [Table 3], [Table 4]

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