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| ORIGINAL/BRIEF |
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| Year : 2012 | Volume
: 4
| Issue : 5 | Page : 60-61 |
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Preparation and formulation of transferosomes containing an antifungal agent for transdermal delivery: Application of Plackett-Burman design to identify significant factors influencing vesicle size
B Rutu Patel, RH Parikh
Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Changa, Gujarat, India
| Date of Web Publication | 21-Mar-2012 |
Correspondence Address:
B Rutu Patel
Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Changa, Gujarat
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0975-7406.94140
 Abstract | | |
Transferosomes containing an anti-fungal agent were prepared by Rotary Flask Evaporation -Sonication method. Eight batches were prepared in triplicate and vesicle size of each batch was determined. Plackett-Burman Design was employed to identify significant formulation and process parameters affecting vesicle size. The amount of lipid and surfactant, volume of ethanol and hydration medium as well as hydration time significantly affect the vesicle size. Keywords: Transferosomes, ultradeformable vesicles, vesicle size
How to cite this article:
Patel B R, Parikh R H. Preparation and formulation of transferosomes containing an antifungal agent for transdermal delivery: Application of Plackett-Burman design to identify significant factors influencing vesicle size. J Pharm Bioall Sci 2012;4, Suppl S1:60-1 |
How to cite this URL:
Patel B R, Parikh R H. Preparation and formulation of transferosomes containing an antifungal agent for transdermal delivery: Application of Plackett-Burman design to identify significant factors influencing vesicle size. J Pharm Bioall Sci [serial online] 2012 [cited 2022 Aug 12];4, Suppl S1:60-1. Available from: https://www.jpbsonline.org/text.asp?2012/4/5/60/94140 |
Transferosomes are also known as Ultradeformable vesicles, Ultradeformable Liposomes or Elastic Vesicles. Transfersome can squeeze itself through a skin pore which is many times smaller than its size owing to its elasticity. Thus, they can increase the skin penetration when applied non-occlusively. The objective of the present study was to identify significant factors influencing vesicle size through the use of Plackett-Burman Design.
| Materials and Methods | |  |
Preparation of transferosomes
They were prepared by Rotary Flask Evaporation -Sonication Method reported by Gregor et al. [1] Soya phosphatidyl choline, Tween80 and antifungal agent were dissolved in Ethanol. Ethanol was evaporated by flask rotation to deposit the film on the wall of the round bottom flask. The film was hydrated at 45°C using Saline phosphate buffer, pH 6.4 with mild agitation. The Transferosomes formed were allowed to swell for 2 hour at room temperature. Vesicles were subsequently sonicated for 30minutes using bath sonicator. (Model: EN-60US,Make: ELECTROQUIP). Eight batches were prepared as per the Plackett - Burman Design [Table 1]. | Table 1: Composition of transferosomes of different batches and their vesicle size
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Measurement of vesicle size
Vesicle size was determined using particle size analyzer employing Dynamic Light Scattering Technique (Model: Nano S90, Make: Malvern, USA). Data analysis was carried out using DOE ++ Software (Version 1.0.7.2 ID -DS-1, ReliaSoft Corporation, USA).
| Result and Discussion | | |
Using Pareto chart [Figure 1] significant and non-significant factors influencing vesicle size were identified. It is evident from the Pareto Chart [Figure 1] as well as P value [Table 1] with respect to vesicle size associated with every variable that the amount of Lipid, amount of Surfactant, volume of Ethanol, volume of Hydration medium and Hydration Time significantly affect the vesicle size.
| Conclusion | | |
From large number of formulation and process variables involved in making of transferosomes, Plackett - Burman Design can help in identifying significant factors influencing vesicle size. These significant factors may be optimized by employing full factorial design. While employing full factorial design, non-significant factors may be fixed at appropriate level (-1 or +1) employed during Plackett - Burman Design.
| Acknowledgement | | |
We are very thankful to Halcyon Labs Pvt. Ltd., Ahmedabad for providing an ANTIFUNGAL AGENT as a gratis sample.[7]
| References | | |
| 1. | Gregor C. Rational design of new product candidates: The next generation of highly deformable bilayer vesicles for noninvasive, targeted therapy. Journal of Controlled Release 2012; Article in press. 
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| 2. | Hardevinder Pal Singh, P. U., Ashok Kumar Tiwary,and Subheet Jain, Elastic Liposomal Formulation for Sustained Delivery of Colchicine: in vitro Characterization and in vivo Evaluation of Anti-gout Activity. The AAPS Journal 2009: 11: 54-64.
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| 3. | P. Loan Honeywell-Nguyen, J. A. B., T he in vitro transport of pergolide from surfactant-based elastic vesicles through human skin: A suggested mechanism of action. Journal of Controlled Release 2003: 86: 145-156.
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| 4. | A. Gillet, A. G., P. Compèrec, B. Evrarda, G. Piel Development of a new topical system: Drug-in-cyclodextrin-in-deformable liposome. International Journal of Pharmaceutics 2009: 380: 174-180.
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| 5. | Gamal M.M. El Maghraby, A. C. W., Brian W. Barry, Oestradiol skin delivery from ultradeformable liposomes: refinement of surfactant concentration. International Journal of Pharmaceutics 2000: 196: 63-74.
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| 7. | Gregor Cevc, G. B., New, highly efficient formulation of diclofenac for the topical,transdermal administration in ultradeformable drug carriers,Transfersomes. Biochimica et Biophysica Acta, 2001:1514:191-205.
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[Figure 1]
[Table 1]
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