|Year : 2015 | Volume
| Issue : 2 | Page : 145-150
Effects of methanolic extracts from edible plants on endogenous secretory receptor for advanced glycation end products induced by the high glucose incubation in human endothelial cells
Yoshinori Okada1, Mizue Okada2
1 Laboratory on Ageing and Health Management, Graduate School of Nursing and Health, Aichi Prefectural University, Nagoya, Japan
2 Nutrition Section, Ageing and Nutrition Research, Yms Laboratory, Gifu, Japan
|Date of Submission||18-Nov-2013|
|Date of Decision||19-Aug-2014|
|Date of Acceptance||23-Sep-2014|
|Date of Web Publication||1-Apr-2015|
Dr. Yoshinori Okada
Laboratory on Ageing and Health Management, Graduate School of Nursing and Health, Aichi Prefectural University, Nagoya
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: In diabetic populations, endogenous secretory receptor for advanced glycation end products (esRAGE) levels may be related to the degree of diabetic complications or to the protection from diabetic complications. Objective: We investigated the impact of 29 methanolic extracts from edible plants on esRAGE production in human umbilical vein endothelial cells (HUVECs) cultured in high (4.5 g/L) glucose. Materials and Methods: Edible plants were minced, and extracts were obtained with methanol overnight. The methanolic extracts from 29 edible plants were evaporated in a vacuum. For screening study purposes, HUVECs were seeded in culture dishes (1.5 × 10 5 cells). Then, HUVECs were incubated with 1 g/L or 4.5 g/L of glucose in SFM CS-C medium treated with methanolic extracts from edible plants (MEEP) for 96 h. Determination of esRAGE production in the cell culture-derived supernatants was performed by colorimetric ELISA. The 8-hydroxydeoxyguanosine (8-OHdG) level was determined by using the 8-OHdG Check ELISA kit. Peroxynitrite-dependent oxidation of 2', 7'- dichlorodihydrofluorescein to 2', 7'-dichlorofluorescein was estimated based on the method described by Crow. Because MEEP were methanolic extracts, we measured their total phenolic content (TPC). TPC was measured with a modified version of the Folin-Ciocalteu method. Results: The results showed eight extracts increased esRAGE production. The extract from white radish sprouts showed the highest esRAGE production activity, and then eggplant, carrot peel, young sweet corn, Jew's marrow, broad bean, Japanese radish and cauliflower. In order to understand the mechanism of esRAGE production, the eight extracts were examined for DNA damage, peroxynitrite scavenging activity, and TPC in correlation with their esRAGE production. The results showed esRAGE production correlates with the peroxynitrite level and TPC. Conclusion: This study supports the utilization of these eight extracts in folk medicine for improved treatment of diabetic complications.
Keywords: 8-hydroxydeoxyguanosine, advanced glycation end products, edible plants, endogenous secretory receptor for advanced glycation end products, peroxynitrite radical scavenging activity, total phenolic content
|How to cite this article:|
Okada Y, Okada M. Effects of methanolic extracts from edible plants on endogenous secretory receptor for advanced glycation end products induced by the high glucose incubation in human endothelial cells. J Pharm Bioall Sci 2015;7:145-50
|How to cite this URL:|
Okada Y, Okada M. Effects of methanolic extracts from edible plants on endogenous secretory receptor for advanced glycation end products induced by the high glucose incubation in human endothelial cells. J Pharm Bioall Sci [serial online] 2015 [cited 2021 Apr 18];7:145-50. Available from: https://www.jpbsonline.org/text.asp?2015/7/2/145/148783
The receptor for advanced glycation end products (RAGE) is a member of the immunoglobulin superfamily and a multiligand receptor for the late products of nonenzymatic glycation (advanced glycation end products [AGEs]). RAGE is also known to be involved in microvascular complications in diabetes through oxidative stress generation, regulation of atherogenesis, the angiogenic response, and vascular injury. ,, Numerous truncated forms of RAGE have been reported, and the C-terminally truncated soluble form of RAGE (sRAGE) has received much attention. Yonekura et al.  found that human vascular cells expressed a novel splice variant coding for an sRAGE protein and named it endogenous secretory receptor for advanced glycation end products (esRAGE). The esRAGE neutralizes AGE action on endothelial cells. In fact, RAGE antagonists are in clinical development as therapeutics for diabetes complications. Interfering with the activation of RAGE, using sRAGE, has been shown to prevent or ameliorate the vascular complications in experimental studies.  Furthermore, in diabetic populations, sRAGE and esRAGE levels may be related to the degree of diabetic complications or to the protection from diabetic complications. Indeed, Ramasamy et al.  first tested sRAGE as a ligand decoy and showed that administration of sRAGE to diabetic animals was at least partially protective against macro- and micro-vascular complications of diabetes. There is the following study about similar diabetic patients. Piarulli et al.  conducted to examine the relationship between esRAGE and oxidative stress in type 2 diabetic mellitus (T2DM) patients with/without advanced macro-angiopathy. They showed that the study established a relationship between esRAGE and oxidative stress and/or antioxidant power, suggesting that esRAGE upregulation might be part of the cell's antioxidative defenses against plaque forming as a result of oxidative stress in the T2DM phenotype. An important question to address, therefore, is the usefulness of circulating sRAGE and esRAGE as potential biomarkers of diabetic complications and need for therapy. However, relatively little is known about factors (thiazolidinedione,  atorvastatin,  and insulin  ) that influence esRAGE production.
The potential beneficial roles of dietary antioxidants have been emphasized in treatment of various diseases including diabetes. Plant and plant-derived products in foods contain antioxidants that are thought to provide health benefits in decreasing the risk of disease. Extensive studies have been focused on the positive role of plant polyphenols as free radical scavengers and in disease prevention. , Polyphenols such as (-)-epigallocatechin-3-gallate (EGCG) and genistein have been reported to possess therapeutic effects for obesity, diabetes, and cardiovascular diseases.  Lee and Lee  investigated the protective effects of EGCG against AGEs-induced damage in neuron cells. The results showed that EGCG was found to down-regulate the mRNA level of RAGE in cells. On the other hand, Jung et al.  indicated that genistein disrupted AGE-RAGE binding and showed genistein exerted inhibitory effects on AGE formation in vitro.
No study has examined phenolic compound influence on esRAGE. However, because methanolic extracts from edible plants (MEEP) were methanolic extracts, we measured their total phenolic content (TPC). Some reports show esRAGE and RAGE to be related to guanosine in DNA , and peroxynitrite radicals.  Therefore, we examined the correlation between esRAGE production and the three indicators mentioned above to determine the mechanism for increasing esRAGE production in human umbilical vein endothelial cells (HUVECs) treated with eight plant extracts.
| Materials and Methods|| |
Preparation of methanolic extracts from 29 edible plants
Edible plants were purchased from an outdoor market, and then shelled or peeled. Edible plants (100 g) were minced into 5 mm fragments and extracts were obtained with methanol (200 mL) overnight at room temperature. The extracts were filtered and the residue was re-extracted under the same conditions as above. The combined filtrates were evaporated in a vacuum slightly below 40°C in a rotary evaporator. MEEP were prepared as a solution in dimethyl sulfoxide (DMSO). The specimen was dissolved in DMSO (1.0 mL).
Human umbilical vein endothelial cells (No. JCRB0408, Health Science Research Resources Bank, Osaka, Japan) were cultured at 37°C in SFM CS-C medium (DS Pharma Biomedical, Osaka, Japan) supplemented with 1.5 g/mL Fungizone under air-CO 2 (95:5) (referred to hereafter as CS-CF medium). For screening study purposes, HUVECs were seeded in 60 mm culture dishes (1.5 × 10 5 cells). Then, HUVECs were incubated with 1 g/L (normal glucose medium) or 4.5 g/L (high glucose medium) of glucose in CS-CF medium treated with MEEP for 96 h. Two groups of experiments were formed, one receiving 1) continuous 10 μL MEEP, and the other receiving 2) continuous 100 μL MEEP.
Measurement of endogenous secretory receptor for advanced glycation end products concentrations in culture media
Determination of esRAGE production in the cell culture-derived supernatants was performed by colorimetric ELISA under the manufacturer's protocol (B-Bridge International, Inc., Tokyo, Japan). Supernatants cultured with each sample were added to ELISA plates to measure the esRAGE content. Plates were analyzed at A 450nm .
Measurement of 8-hydroxydeoxyguanosine in human umbilical vein endothelial cells DNA
DNA of HUVECs was extracted from the homogenates using a DNA Extractor WB kit (Wako Pure Chemical Industries, Osaka, Japan) according to the protocol. The 8-OHdG level was determined by using the 8-OHdG Check ELISA kit (Japan Institute for the Control of Aging, Shizuoka, Japan) according to the manufacturer's protocol. A 450nm was measured using a microplate reader. The recovery rate of 8-OHdG level was expressed as % recovery of the normal glucose medium A 450nm (experimental A 450nm /normal glucose medium A 450nm × 100).
Measurement of peroxynitrite level
Peroxynitrite-dependent oxidation of 2,7- dichlorodihydrofluorescein (DCDHF) to 2',7'-dichlorofluorescein (DCF) was estimated based on the method described by Crow.  Samples or control (DMSO) were added to 100 mM PBS (pH 7.4) containing 100 μM diethylenetriamine-N, N, N', N", N"-pentaacetic acid (Dojindo Molecular Technologies, Inc., Japan), 5 μM sodium peroxynitrite and 100 μM DCDHF. The mixture was then incubated for 5 min at 37°C. The absorbance was measured at 500 nm, which is the absorbance of DCF. The peroxynitrite scavenging activity was expressed as % inhibition of the control A 500nm ([control A 500nm − experimental A 500nm ]/control A 500nm × 100).
Determination of total phenolic content in methanolic extracts from edible plants
Because MEEP were methanolic extracts, we measured their TPC. TPC was measured with a modified version of the Folin-Ciocalteu method  using chlorogenic acid as a standard. Briefly, 100 μL of the sample or standard were combined with 200 μL of Folin-Ciocalteu reagent and 2.0 mL of 2% Na 2 CO 3 solution. We allowed the mixture to sit for 60 min before reading absorbance at 750 nm using a UV-460 spectrophotometer (Nihonbunko Ltd., Tokyo, Japan). The concentration of TPC present in MEEP was determined by comparing it with the absorbance rates of standard chlorogenic acid at different concentrations.
We present all data as mean ± standard deviation. A statistical comparison between the groups was carried out using either ANOVA or Student's t-test. P <0.10 were considered as statistically significant.
| Results and Discussion|| |
Effects of methanolic extracts from edible plants on endogenous secretory receptor for advanced glycation end products production in high glucose-induced human umbilical vein endothelial cells
We examined the esRAGE-producing ability of 29 MEEP in high glucose-induced HUVECs. The result showed an increase in esRAGE production in the following gight samples (eggplant [Solanum melongena], carrot peel [Daucus carota subsp. sativus], young sweet corn [Zea mays saccharata], broad bean [Vicia faba], Japanese radish [Raphanus sativus var. longipinnatus], white radish [R. sativus var. longipinnatus] sprout, Jew's marrow [Corchorus olitorius] and cauliflower [Brassica oleracea var. botrytis]). The results of the esRAGE production of these eight samples on HUVECs are summarized in [Figure 1]. [Figure 1]a shows the effects of the eight samples (10 μL) on esRAGE production in high glucose-induced HUVECs. The esRAGE production resulting from high glucose treatment was induced when cells were treated with 8 plant extracts as compared to control treated with 4.5 g/L of glucose only. Genistein (10 μM) increased esRAGE production by approximately 12%, but EGCG (10 μM) caused no increase. The results for plant samples suggest that white radish sprout, Jew's marrow and cauliflower have esRAGE production potential on induced endothelial cells. In particular, white radish sprout, which had higher esRAGE production potential than genistein, significantly (P < 0.01) increased esRAGE production. In addition, white radish sprout showed an esRAGE production potential approximately 2.4 times higher than the control (4.5 g/L of glucose only). On the other hand, in 100 μL MEEP [Figure 1]b, more samples had a significant influence on esRAGE production. The results suggest that eggplant, carrot peel, young sweet corn, broad bean, Japanese radish, and white radish sprout have esRAGE production potential on induced endothelial cells. However, different MEEP seem to have different effects on esRAGE production in the presence of high glucose. In 100 μM concentration, genistein also showed a tendency to increase slightly, but the increase was not seen in EGCG. In studies to date, no plant has yet been shown to increase esRAGE production. Therefore, the mechanism by which white radish sprout and the seven other kinds of plants increased esRAGE production is unclear. However, it was reported through similar experimental techniques that Hibiscus sabdariffa polyphenolic extract inhibited the expression of RAGE.  Furthermore, Lu et al.  indicated that atorvastatin exerted a beneficial effect on diabetic nephropathy with reduced AGE accumulation, down-regulating RAGE expression and up-regulating sRAGE in the kidney. From the above-mentioned report, the increase in esRAGE production may be considered to result from the down-regulation of RAGE by the polyphenolic extract. However, although Lee and Lee  show that 5 μM EGCG down-regulates the RAGE expression, this may not necessarily indicate an inverse correlation between RAGE and esRAGE because in our study 10 μM EGCG does not increase esRAGE production. This supposition requires further study.
|Figure 1: Effect of methanolic extracts from eight plants on endogenous secretory receptor for advanced glycation end products (esRAGE) production in human umbilical vein endothelial cells cultured in high glucose. The esRAGE productions of control (dimethyl sulfoxide) and samples in human umbilical vein endothelial cells cultured in high (4.5 g/L) glucose are indicated by unshaded and shaded columns, respectively. Two groups of experiments were formed, one receiving continuous 10 μL methanolic extracts from edible plants (MEEP) (a) and the other receiving continuous 100 μL MEEP (b) Values are the mean ± standard deviation of three measurements. **P < 0.01, *P < 0.05 compared with the controls|
Click here to view
Receptor for advanced glycation end products plays the essential role in the pathogenesis of diabetic vascular complications. Skrha et al.  performed the study to compare concentration of sRAGE and its ligands (EN-RAGE and HMGB1) in type 1 (T1DM) and T2DM. Serum sRAGE concentration was higher in T1DM as compared to controls. Similarly, EN-RAGE was significantly higher in both diabetic groups and HMGB1 concentrations were elevated in T2DM patients. They concluded that serum sRAGE and RAGE ligands concentrations reflect endothelial dysfunction developing in diabetes. Therefore, the extract of these eight plants may have an effect on T2DM disease restraint through esRAGE production.
On the other hand, some reports show esRAGE and RAGE to be related to guanosine in DNA , and peroxynitrite radicals.  On other word, Ohe et al.  showed that mutagenesis of the G-rich cis-elements caused an increase in the esRAGE/membrane-bound RAGE ratio in the minigene-transfected cells. In addition, Liu et al.  showed that administration of EUK134 (peroxynitrite scavenger) attenuated RAGE expression, and decreased cardiomyocyte apoptosis in diabetic mice. Therefore, we examined the correlation between esRAGE production, and the two indicators mentioned above (peroxynitrite and 8-OHdG levels) to determine the mechanism for increasing esRAGE production in HUVECs treated with a eight plant extracts.
Assessment of the relationship between 8-hydroxydeoxyguanosine level and endogenous secretory receptor for advanced glycation end products production
Deoxyribose was used as a detector molecule to identify radical damage. Oyama et al.  showed that the amount of 8-OHdG in the medium increased to a significantly greater extent in high glucose-incubated HUVECs than in low glucose-incubated HUVECs. In our experiment, significant differences were observed for 8-OHdG levels among the eight plant varieties. The level of 8-OHdG was in the range of 0.28-0.52 ng/mL (data not shown). Mizutani et al.  showed that genistein reduced AGEs-induced 8-OHdG formation. The formation of 8-OHdG in calf thymus DNA by 3-morpholinosydnomine N-ethylcarbamide (SIN-1; peroxynitrite donor) was also inhibited by EGCG.  In our experiment, EGCG (100 μM) showed 8-OHdG recovery rates of approximately 90%, but genistein (100 μM) caused 8-OHdG recovery rates of approximately 60%. The calculated 8-OHdG recovery rates for white radish sprout, broad bean, young sweet corn, Japanese radish, eggplant, Jew's marrow, cauliflower, and carrot peel were 61.9%, 47.7%, 68.9%, 55.5%, 43.8%, 62.0%, 60.2%, and 77.1%, respectively in comparison to the normal glucose medium [Figure 2] indicating that the samples are potent inhibitors of 8-OHdG. In particular, carrot peel and young sweet corn which had higher 8-OHdG recovery rate potential than genistein, significantly (P < 0.05) decreased 8-OHdG level. In addition, carrot peel showed an 8-OHdG recovery rate in the vicinity of EGCG. [Figure 3] shows the correlation between esRAGE production and 8-OHdG level of eight plant extracts. The results show that the 8-OHdG level does not correlate with esRAGE production (r = −0.086).
|Figure 2: The calculated 8-hydroxydeoxyguanosine (8-OHdG) recovery rates of methanolic extracts from eight plants. The 8-OHdG recovery rates of control (normal glucose medium) and samples (high glucose medium) are indicated by unshaded and shaded columns, respectively. Values are the mean ± standard deviation of three measurements. **P < 0.01, *P < 0.05 compared with the controls.|
Click here to view
|Figure 3: The correlation between endogenous secretory receptor for advanced glycation end products production and 8-hydroxydeoxyguanosine level of methanolic extracts from eight plants|
Click here to view
Assessment of the relationship between peroxynitrite level and endogenous secretory receptor for advanced glycation end products production
[Figure 4] shows the calculated peroxynitrite scavenging activities of eight extracts. Because peroxynitrite has a short half-life, the measurement of the intracellular scavenging activity is difficult. Therefore, we performed the scavenging activity measurement of the sample in the test tube. Amongst the eight extracts, white radish sprout revealed the highest scavenging activity (44.8%). However, eggplant and broad bean did not show this activity. [Figure 5] shows the correlation between esRAGE production and peroxynitrite level of the eight plant extracts. The peroxynitrite level significantly correlated with esRAGE production (r = -0.706, P < 0.10). The results showed a negative correlation between esRAGE production and peroxynitrite level. Wu et al.  showed that treatment of sinoaortic denervated rats with sRAGE abated aortic oxidative stress. This was marked by reduction in the formation of peroxynitrite indicating that there may be an association between peroxynitrite and esRAGE.
|Figure 4: The calculated peroxynitrite scavenging activities of eight extracts in the test tube. Values are the mean ± standard deviation of three measurements. **P < 0.01, *P < 0.05 compared with the controls|
Click here to view
|Figure 5: The correlation between endogenous secretory receptor for advanced glycation end products production and peroxynitrite level of methanolic extracts from eight plants|
Click here to view
Assessment of the relationship between total phenolic content and endogenous secretory receptor for advanced glycation end products production
The correlation between esRAGE production and TPC was analyzed [Figure 6]. The TPC of the eight MEEP significantly correlated with esRAGE production. The correlation coefficient for esRAGE production was found to be greater than 0.6 (r = 0.660, P < 0.10). A positive correlation between esRAGE production and TPC was found in the eight MEEP. This result proved that the TPC of these plants could be clearly attributed to their esRAGE production.
|Figure 6: The correlation between endogenous secretory receptor for advanced glycation end products production and total phenolic content of methanolic extracts from eight plants|
Click here to view
The molecular mechanisms underlying these effects are unknown. However, the seven MEEP ,,,,,, except for eggplant contain a lot of quercetin. Because there was a positive correlation between TPC and esRAGE [Figure 6], it is very likely to be quercetin that had an effect. The effect of these seven plants may resemble the mechanism that Zhang et al.  showed. Hyperoside, quercetin-3-O-galactoside, is a flavonoid isolated from many medicinal plants. In their studies, quiescent ECV304 (human endothelial-like) cells were treated in vitro with advanced glycation end products (AGEs) in the presence or absence of hyperoside. The results demonstrated that AGEs induced c-Jun N-terminal kinases (JNK) activation and apoptosis in ECV304 cells. Hyperoside inhibited these effects and promoted ECV304 cell proliferation. Furthermore, hyperoside significantly inhibited RAGE expression in AGE-stimulated ECV304 cells, whereas knockdown of RAGE inhibited AGE-induced JNK activation. These results suggested that AGEs may promote JNK activation, leading to viability inhibition of ECV304 cells via the RAGE signaling pathway. These effects could be inhibited by hyperoside. Furthermore, from the above-mentioned report,  the increase in esRAGE production may be considered a result of the down-regulation of RAGE by the polyphenolic extract. Our findings suggest a novel role for eight edible plants in the treatment and prevention of diabetes.
| Acknowledgments|| |
Research was supported by a Grant-in-Aid for Scientific Research (Grant No. 18650218) from the Japanese Ministry of Education, Culture, Sports, Science and Technology.
| References|| |
Chawla D, Bansal S, Banerjee BD, Madhu SV, Kalra OP, Tripathi AK. Role of advanced glycation end product (AGE)-induced receptor (RAGE) expression in diabetic vascular complications. Microvasc Res 2014;95C:1-6.
Li W, Xu H, Hu Y, He P, Ni Z, Xu H, et al.
Edaravone protected human brain microvascular endothelial cells from methylglyoxal-induced injury by inhibiting AGEs/RAGE/oxidative stress. PLoS One 2013;8:e76025.
Stirban A, Gawlowski T, Roden M. Vascular effects of advanced glycation endproducts: Clinical effects and molecular mechanisms. Mol Metab 2014;3:94-108.
Yonekura H, Yamamoto Y, Sakurai S, Watanabe T, Yamamoto H. Roles of the receptor for advanced glycation endproducts in diabetes-induced vascular injury. J Pharmacol Sci 2005;97:305-11.
Lee D, Lee KH, Park H, Kim SH, Jin T, Cho S, et al.
The effect of soluble RAGE on inhibition of angiotensin II-mediated atherosclerosis in apolipoprotein E deficient mice. PLoS One 2013;8:e69669.
Ramasamy R, Yan SF, Schmidt AM. The diverse ligand repertoire of the receptor for advanced glycation endproducts and pathways to the complications of diabetes. Vascul Pharmacol 2012;57:160-7.
Piarulli F, Lapolla A, Ragazzi E, Susana A, Sechi A, Nollino L, et al.
Role of endogenous secretory RAGE (esRAGE) in defending against plaque formation induced by oxidative stress in type 2 diabetic patients. Atherosclerosis 2013;226:252-7.
Tan KC, Chow WS, Tso AW, Xu A, Tse HF, Hoo RL, et al.
Thiazolidinedione increases serum soluble receptor for advanced glycation end-products in type 2 diabetes. Diabetologia 2007;50:1819-25.
Tam HL, Shiu SW, Wong Y, Chow WS, Betteridge DJ, Tan KC. Effects of atorvastatin on serum soluble receptors for advanced glycation end-products in type 2 diabetes. Atherosclerosis 2010;209:173-7.
Lam JK, Wang Y, Shiu SW, Wong Y, Betteridge DJ, Tan KC. Effect of insulin on the soluble receptor for advanced glycation end products (RAGE). Diabet Med 2013;30:702-9.
Schini-Kerth VB, Auger C, Kim JH, Etienne-Selloum N, Chataigneau T. Nutritional improvement of the endothelial control of vascular tone by polyphenols: Role of NO and EDHF. Pflugers Arch 2010;459:853-62.
Khattak KF. Nutrient composition, phenolic content and free radical scavenging activity of some uncommon vegetables of Pakistan. Pak J Pharm Sci 2011;24:277-83.
Hwang JT, Park IJ, Shin JI, Lee YK, Lee SK, Baik HW, et al.
Genistein, EGCG, and capsaicin inhibit adipocyte differentiation process via activating AMP-activated protein kinase. Biochem Biophys Res Commun 2005;338:694-9.
Lee SJ, Lee KW. Protective effect of (-)-epigallocatechin gallate against advanced glycation endproducts-induced injury in neuronal cells. Biol Pharm Bull 2007;30:1369-73.
Jung DH, Kim YS, Kim JS. Screening system of blocking agents of the receptor for advanced glycation endproducts in cells using fluorescence. Biol Pharm Bull 2012;35:1826-30.
Ohe K, Watanabe T, Harada S, Munesue S, Yamamoto Y, Yonekura H, et al.
Regulation of alternative splicing of the receptor for advanced glycation endproducts (RAGE) through G-rich cis-elements and heterogenous nuclear ribonucleoprotein H. J Biochem 2010;147:651-9.
Fan Q, Liao J, Kobayashi M, Yamashita M, Gu L, Gohda T, et al.
Candesartan reduced advanced glycation end-products accumulation and diminished nitro-oxidative stress in type 2 diabetic KK/Ta mice. Nephrol Dial Transplant 2004;19:3012-20.
Liu Y, Qu Y, Wang R, Ma Y, Xia C, Gao C, et al.
The alternative crosstalk between RAGE and nitrative thioredoxin inactivation during diabetic myocardial ischemia-reperfusion injury. Am J Physiol Endocrinol Metab 2012;303:E841-52.
Crow JP. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro
: Implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide 1997;1:145-57.
Gao X, Ohlander M, Jeppsson N, Björk L, Trajkovski V. Changes in antioxidant effects and their relationship to phytonutrients in fruits of sea buckthorn (Hippophae rhamnoides
L.) during maturation. J Agric Food Chem 2000;48:1485-90.
Peng CH, Chyau CC, Chan KC, Chan TH, Wang CJ, Huang CN. Hibiscus sabdariffa
polyphenolic extract inhibits hyperglycemia, hyperlipidemia, and glycation-oxidative stress while improving insulin resistance. J Agric Food Chem 2011;59:9901-9.
Lu L, Peng WH, Wang W, Wang LJ, Chen QJ, Shen WF. Effects of atorvastatin on progression of diabetic nephropathy and local RAGE and soluble RAGE expressions in rats. J Zhejiang Univ Sci B 2011;12:652-9.
Skrha J Jr, Kalousová M, Svarcová J, Muravská A, Kvasnicka J, Landová L, et al.
Relationship of soluble RAGE and RAGE ligands HMGB1 and EN-RAGE to endothelial dysfunction in type 1 and type 2 diabetes mellitus. Exp Clin Endocrinol Diabetes 2012;120:277-81.
Oyama T, Miyasita Y, Watanabe H, Shirai K. The role of polyol pathway in high glucose-induced endothelial cell damages. Diabetes Res Clin Pract 2006;73:227-34.
Mizutani K, Ikeda K, Nishikata T, Yamori Y. Phytoestrogens attenuate oxidative DNA damage in vascular smooth muscle cells from stroke-prone spontaneously hypertensive rats. J Hypertens 2000;18:1833-40.
Moon HK, Yang ES, Park JW. Protection of peroxynitrite-induced DNA damage by dietary antioxidants. Arch Pharm Res 2006;29:213-7.
Wu F, Feng JZ, Qiu YH, Yu FB, Zhang JZ, Zhou W, et al.
Activation of receptor for advanced glycation end products contributes to aortic remodeling and endothelial dysfunction in sinoaortic denervated rats. Atherosclerosis 2013;229:287-94.
Hashimoto T, Ueda Y, Oi N, Sakakibara H, Piao C, Ashida H, et al.
Effects of combined administration of quercetin, rutin, and extract of white radish sprout rich in kaempferol glycosides on the metabolism in rats. Biosci Biotechnol Biochem 2006;70:279-81.
Ahmed FA, Ali RF. Bioactive compounds and antioxidant activity of fresh and processed white cauliflower. Biomed Res Int 2013;2013:367819.
Azuma K, Nakayama M, Koshioka M, Ippoushi K, Yamaguchi Y, Kohata K, et al.
Phenolic antioxidants from the leaves of Corchorus olitorius
L. J Agric Food Chem 1999;47:3963-6.
Poulin MJ, Bel-Rhlid R, Piché Y, Chênevert R. Flavonoids released by carrot (Daucus carota
) seedlings stimulate hyphal development of vesicular-arbuscular mycorrhizal fungi in the presence of optimal CO2 enrichment. J Chem Ecol 1993;19:2317-27.
Rozema J, Björn LO, Bornman JF, Gaberscik A, Häder DP, Trost T, et al.
The role of UV-B radiation in aquatic and terrestrial ecosystems - An experimental and functional analysis of the evolution of UV-absorbing compounds. J Photochem Photobiol B 2002;66:2-12.
Biesaga M. Influence of extraction methods on stability of flavonoids. J Chromatogr A 2011;1218:2505-12.
Beevi SS, Narasu ML, Gowda BB. Polyphenolics profile, antioxidant and radical scavenging activity of leaves and stem of Raphanus sativus
L. Plant Foods Hum Nutr 2010;65:8-17.
Zhang Z, Sethiel MS, Shen W, Liao S, Zou Y. Hyperoside downregulates the receptor for advanced glycation end products (RAGE) and promotes proliferation in ECV304 cells via the c-Jun N-terminal kinases (JNK) pathway following stimulation by advanced glycation end-products in vitro
. Int J Mol Sci 2013;14:22697-707.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]
|This article has been cited by|
||Mangostanaxanthones III and IV: advanced glycation end-product inhibitors from the pericarp of Garcinia mangostana
| ||Hossam M. Abdallah,Hany M. El-Bassossy,Gamal A. Mohamed,Ali M. El-Halawany,Khalid Z. Alshali,Zainy M. Banjar |
| ||Journal of Natural Medicines. 2017; 71(1): 216 |
|[Pubmed] | [DOI]|
||Phenolics from Garcinia mangostana Inhibit Advanced Glycation Endproducts Formation: Effect on Amadori Products, Cross-Linked Structures and Protein Thiols
| ||Hossam Abdallah,Hany El-Bassossy,Gamal Mohamed,Ali El-Halawany,Khalid Alshali,Zainy Banjar |
| ||Molecules. 2016; 21(2): 251 |
|[Pubmed] | [DOI]|
||Potential Properties of Plant Sprout Extracts on Amyloid▀
| ||Mizue Okada,Yoshinori Okada |
| ||Biochemistry Research International. 2016; 2016: 1 |
|[Pubmed] | [DOI]|