Journal of Pharmacy And Bioallied Sciences
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ORIGINAL ARTICLE
Year : 2017  |  Volume : 9  |  Issue : 1  |  Page : 33-43

Evaluation of Cassia tora Linn. against oxidative stress-induced DNA and cell membrane damage


1 Department of Biotechnology, College of Agriculture, University of Agricultural Sciences, Hassan, Bengaluru, Karnataka, India
2 Department of Studies and Research in Biochemistry, P.G Centre, Mangalore University, Chikka Aluvara, Kodagu, Karnataka, India
3 Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, Karnataka, India

Correspondence Address:
Ramesh Balenahalli Narasingappa
Department of Biotechnology, College of Agriculture, University of Agriculture Sciences, Hassan, Bengaluru - 573 225, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0975-7406.206215

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Objective: The present study aims to evaluate antioxidants and protective role of Cassia tora Linn. against oxidative stress-induced DNA and cell membrane damage. Materials and Methods: The total and profiles of flavonoids were identified and quantified through reversed-phase high-performance liquid chromatography. In vitro antioxidant activity was determined using standard antioxidant assays. The protective role of C. tora extracts against oxidative stress-induced DNA and cell membrane damage was examined by electrophoretic and scanning electron microscopic studies, respectively. Results: The total flavonoid content of CtEA was 106.8±2.8mg/g d.w.QE, CtME was 72.4±1.12mg/g d.w.QE, and CtWE was 30.4±0.8mg/g d.w.QE. The concentration of flavonoids present in CtEA in decreasing order: quercetin>kaempferol>epicatechin; in CtME: quercetin>rutin>kaempferol; whereas, in CtWE: quercetin>rutin>kaempferol. The CtEA inhibited free radical-induced red blood cell hemolysis and cell membrane morphology better than CtME as confirmed by a scanning electron micrograph. CtEA also showed better protection than CtME and CtWE against free radical-induced DNA damage as confirmed by electrophoresis. Conclusion: C. tora contains flavonoids and inhibits oxidative stress and can be used for many health benefits and pharmacotherapy.


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