Journal of Pharmacy And Bioallied Sciences
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Year : 2018  |  Volume : 10  |  Issue : 1  |  Page : 43-47

Initial response of human bone marrow–derived stem cells after contact with ultrahigh-molecular-weight polyethylene (UHMWPE) material: An in vitro study on cell viability and interleukin-6 expression

1 Orthopaedic and Traumatology Department, Prof. Dr. R. Soeharso Orthopaedic Hospital, Surakarta, Indonesia
2 Laboratory for Engineering Design and Tribology, Department of Mechanical Engineering, Diponegoro University, Tembalang, Semarang, Indonesia
3 Laboratory for Surface Technology and Tribology, Faculty of Engineering Technology, University of Twente Drienerloolaan, Enschede, The Netherlands

Correspondence Address:
Dr. Iwan B Anwar
Orthopaedic and Traumatology Department, Prof. Dr. R. Soeharso Orthopaedic Hospital, Jl. A. Yani Pabelan, Surakarta 57162
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jpbs.JPBS_70_17

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Introduction: Ultrahigh-molecular-weight polyethylene (UHMWPE) is a thermoplastic polymer useful in biomaterial applications, especially in orthopedic field. Yet, little is known concerning its initial effect on human bone marrow stem cells (hBMSCs) after implantation. Materials and Methods: A cytotoxicity analysis was performed with a 3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium assay after 24, 48, and 72h of incubation of hBMSC culture. Expression of interleukin-6 (IL-6) was measured using enzyme-linked immunosorbent assay. Cell viability was measured with Inhibitory concentration 50% (IC50) formula. Results: All treatment groups showed a cell viability of >50% ranging from 78% to >100%. Lower expression of IL-6 of hBMSC compared to control group was found in 48h of incubation period. Conclusion: hBMSC showed high cell viability after initial contact with UHMWPE material. Modulation of IL-6 expression was present at the initial stage as a response to foreign material.

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