| ORIGINAL ARTICLE |
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| Year : 2018 | Volume
: 10
| Issue : 1 | Page : 48-53 |
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Hypnotic effect of red cabbage (Brassica oleracea) on pentobarbital-induced sleep in mice
Azar
Hosseini1, Mohammad-Ali
Sobhanifar2, Fatemeh
Forouzanfar2, Azita
Aghaee1, Hassan
Rakhshandeh2
1 Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
2 Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Correspondence Address:
Dr. Hassan
Rakhshandeh
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad
Iran
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/jpbs.JPBS_215_17
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Objective: The present study was performed to investigate the effect of hydroalcoholic extract of red cabbage and its fractions on sleeping behavior in mice. Materials and Methods: The extract and its fractions were injected to mice and sleep duration as well as sleep latency were recorded. Furthermore, toxicity of the extract was determined both in vivo and in vitro. Results: The extract increased sleep duration at doses of 50–200mg/kg (P < 0.001). This observed hypnotic effect was comparable to that of diazepam (3mg/kg) (P < 0.001 in comparison with control group). Ethyl acetate, n-butanol, and aqueous fractions could increase sleep duration (P < 0.001). The sleep latency was decreased by the extract (P < 0.001) and only ethyl acetate fraction (P < 0.001). LD50 value for red cabbage extract was 2.4g/kg. There was no toxic effect on viability of cultured neuronal cells (PC12). Rotarod test results showed that there were no significant differences between the extract groups and the control group. Conclusion: The results suggest that red cabbage potentiates pentobarbital hypnosis without any toxic effect. The main component(s) responsible for this effect is most likely to be intermediate polar agent(s) such as flavonoids, which are found in ethyl acetate fraction of this plant.
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