Journal of Pharmacy And Bioallied Sciences
Journal of Pharmacy And Bioallied Sciences Login  | Users Online: 959  Print this pageEmail this pageSmall font sizeDefault font sizeIncrease font size 
    Home | About us | Editorial board | Search | Ahead of print | Current Issue | Past Issues | Instructions | Online submission




 
 Table of Contents  
ORIGINAL ARTICLE
Year : 2021  |  Volume : 13  |  Issue : 5  |  Page : 461-464  

Assessment of age-related changes of salivary immunoglobulin a levels among healthy individuals


1 Department of Oral Medicine and Radiology, Vasantdada Patil Dental College and Hospital, Sangli, Maharashtra, India
2 Department of Dentistry, B.K.L. Walawalkar Rural Medical College, Chiplun, Maharashtra, India
3 Department of Conservative Dentistry and Endodontics, Vasantdada Patil Dental College and Hospital, Sangli, Maharashtra, India
4 Department of Conservative Dentistry and Endodontics, Pandit Deendayal Upadhyay Dental College and Hospital, Solapur, Maharashtra, India
5 Department of Orhtodontics, Aditya Dental College, Beed, Maharashtra, India

Date of Submission27-Sep-2020
Date of Decision27-Sep-2020
Date of Acceptance28-Sep-2020
Date of Web Publication05-Jun-2021

Correspondence Address:
Dipak Baliram Patil
Department of Dentistry, B.K.L. Walawalkar Rural Medical College, Chiplun – Ratnagiri, Maharashtra
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jpbs.JPBS_605_20

Rights and Permissions
   Abstract 


Background: Secretory immunoglobulin A (IgA) is the first line of defense against pathogens that invade mucosal surfaces. It has been reported that the immune system exhibits profound age-related changes. The aim of this study was to investigate the age-dependent changes of salivary IgA among healthy individuals. Materials and Methods: Saliva samples were collected from 120 healthy individuals (aged 11–70 years). The salivary IgA concentrations were measured by the use of a single radial immunodiffusion technique and analyzed using the Mann–Whitney U, Kruskal–Wallis, and Chi-square tests. Results: The mean salivary IgA levels were 81.11 ± 4.50 mg/dl at age 11–20 years, 92.71 ± 13.76 mg/dl at age 21–30 years, 96.50 ± 4.04 mg/dl at age 31–40 years, 104.96 ± 10.15 mg/dl at age 41–50 years, 113.22 ± 7.85 mg/dl at age 51–60 years, and 91.38 ± 4.77 mg/dl at age 61–70 years. There was a significant difference among the mean salivary IgA levels of different age groups (P < 0.001). Conclusion: These results showed that the salivary IgA levels exhibit age-related changes. Oral immunization may be considered to improve oral immunity when the salivary concentrations of IgA begin to decrease during lifetime.

Keywords: Adult, immunoglobulin A, saliva, SRID


How to cite this article:
Shete MD, Patil DB, Karade P, Chopade R, Gandhi N, Alane U. Assessment of age-related changes of salivary immunoglobulin a levels among healthy individuals. J Pharm Bioall Sci 2021;13, Suppl S1:461-4

How to cite this URL:
Shete MD, Patil DB, Karade P, Chopade R, Gandhi N, Alane U. Assessment of age-related changes of salivary immunoglobulin a levels among healthy individuals. J Pharm Bioall Sci [serial online] 2021 [cited 2021 Aug 5];13, Suppl S1:461-4. Available from: https://www.jpbsonline.org/text.asp?2021/13/5/461/317579




   Introduction Top


S ecretory immunoglobulin A (IgA) constitutes the predominant immunoglobulin isotype in secretions, including saliva. It is considered to be the first line of defense of the host against pathogens that colonize or invade mucosal surfaces.[1] Salivary IgA antibodies could help maintain the integrity of the oral surfaces by preventing microbial adherence to epithelial and tooth surfaces, by neutralizing enzymes, toxins, and viruses, or by acting in synergy with other antibacterial factors such as lysozyme, lactoferrin, salivary peroxidase, and mucins.[1],[2] Salivary IgA may also prevent the penetration of food antigens in the oral mucosa. Some studies have also demonstrated that a lower incidence of caries resulted from a high salivary IgA concentration.[3],[4] In addition, a lower concentration of IgA in saliva has been presented as a risk factor for upper respiratory infection in children and the elderly.[5] Furthermore, lower levels of salivary IgA are associated with increased risk for periodontal disease and caries.[6],[7]

However, it has been shown that mucosal immunization is an effective method in increasing the IgA levels in mucosal secretions.[8] Accordingly, it may be possible to induce salivary IgA response via oral immunization, when the salivary IgA concentrations begin to decrease during a lifetime. This strategy could help the maintenance of the salivary IgA levels and improve the oral immunity.


   Materials and Methods Top


The study included 120 healthy individuals visiting the Department of Oral Medicine and Radiology for routine dental health checkups at Vasantdada Patil Dental College and Hospital, Sangli, in various study groups. The study groups were categorized into six different groups – Group I: 11–20 years, Group II: 21–30 years, Group III: 31–40 years, Group IV: 41–50 years, Group V: 51–60 years, and Group VI: 61–70 years. The entire project was submitted to the Ethical Committee of our institute, and the approval was obtained for the study. Informed consent was obtained from parents of school-going children (minor group), and the patients willing to participate in the study were enrolled.

Inclusion criteria

  • Only healthy individuals in the second to seventh decade of their life.


Exclusion criteria

  • Patients with history of any pathology associated with oral mucosa
  • Patients with history of salivary gland disorders
  • Patients with history of tobacco and alcohol consumption
  • Patients with history of systemic illness.


Collection of saliva

The saliva samples were collected between 10 and 11 am. Patients were instructed not to eat or drink for at least before the collection of saliva samples. The patient was asked to sit idle for 5 min and to collect saliva. For saliva collection, participants were asked to generate saliva in their mouths and to spit into sterilized wide containers. The saliva samples were stored in a refrigerator and then transported to the laboratory. In the laboratory, all samples were then centrifuged to 15 min at 1000 rpm to remove cells and debris.

Estimation of salivary immunoglobulin

The quantitative estimation of immunoglobulin IgA was done by a single radial immune diffusion technique.[9] This method consists of radial diffusion of salivary antigen from point application into the antibody-containing gels forming a circular precipitate at the point of equivalence.

Statistical analysis

Differences in variables were analyzed using the Mann–Whitney U, Kruskal-Wallis, and Chi-square tests as appropriate, and P < 0.05 was considered statistically significant.


   Results Top


According to our study, the results of salivary IgA levels in different age groups were as follows: Group 1 (11–20 years) showed values of 81.11 ± 4.50 mg/dl, Group 2 (21–30 years) showed 92.71 ± 13.76 mg/dl, Group 3 (31–40 years) showed 96.50 ± 4.04 mg/dl, Group 4 (41–50 years) showed 104.96 ± 10.15 mg/dl, Group 5 (51–60 years) showed 113.22 ± 7.85 mg/dl, and Group 6 (61–70 years) showed 91.38 ± 4.77 mg/dl [Table 1]. Statistical analysis showed a significant difference among mean salivary IgA levels of different age groups (P < 0.001). These results showed that the mean salivary IgA level increased with increasing age up to 60 years and then decreased in the last age group, i.e., from 61 to 70 years [Graph 1]. Alterations in pattern of salivary IgA were observed in the male and female groups. In each age group, the mean salivary IgA levels tended to be slightly higher in women, although the differences were not statistically significant [Table 2].
Table 1: Comparison of six age groups with respect to IgA levels by Kruskal-Wallis ANOVA

Click here to view
Table 2: Comparison of males and females in six age groups with respect to IgA levels by Mann-Whitney U test

Click here to view




   Discussion Top


Secretory IgA is considered to be the principal mediator of host defense at mucosal surfaces.[1] We hereby described the changes of salivary IgA and IgE levels across different ages from 1 to 70 years. As we observed, the salivary IgA levels increased with increasing age up to 60 years and then decreased. Moreover, the detectable rates and levels of salivary IgA increased from age 1 to 40 years and then decreased. The salivary concentrations of both immunoglobulins were higher in adults compared to children. In some studies, the age-related changes of salivary immunoglobulin concentrations (especially IgA) have been reported. Eliasson et al.[10] investigated the IgA concentrations in secretions of palatal, buccal, and labial salivary glands in individuals aged 18–72 years. They have observed that the salivary IgA concentrations in these saliva samples were higher in elderly individuals (≥65 years) as compared to individuals aged 18–64 years. Increased whole-saliva IgA concentrations in older ages have been attributed partly to positive age-related effects on IgA concentration in the buccal gland secretion. Weemaes et al.[11] reported that the salivary IgA secretion rate increased during infancy and childhood period at age 1–12 years. Childers et al.[12] determined the concentration of IgA in parotid saliva of healthy children (age 6–12 years, n = 14) and healthy adults (age 22–51 years, n = 20) and reported that the levels of IgA increased with age. Challacombe et al.[13] showed in healthy adults that the salivary IgA concentrations increased with age and reached the maximum levels in the oldest studied group (>80 years). These investigations are nearly consistent with our results. However, genetic and environmental differences and differential oral health may account for some different results obtained in this study and those reported by other investigators. For example, in our study, the mean salivary IgA levels decreased after 60 years. Our results regarding decline in salivary IgA concentration after 60 years may be attributed to the increased susceptibility of elderly individuals to oral infectious diseases, especially infection with IgA-degrading bacteria.[1] Regarding salivary IgA changes, it has been reported that the stimulus for IgA synthesis at mucosal surfaces appears to be the colonization of these surfaces by commensal bacteria-bearing polyclonal mitogens such as lipopolysaccharide and perhaps lipoteichoic acid, since germ-free mammals have underdeveloped mucosa-associated lymphoid tissues and lack IgA in their secretions.[14],[15] Although IgA is thought to act to exclude extrinsic pathogenic microorganisms, it appears to be without effect on commensal bacteria, since these microbes colonize and persist on mucosal and tooth surfaces despite its presence.[14] The reasons for this persistence are unknown, although immune tolerance and antigenic variation have been proposed.

The immunological basis of the age-dependent changes in salivary IgA concentrations could be partly explained according to the responses of T-helper (Th) and regulatory T (Treg) cells. Th1 cells are characterized by the secretion of cytokines such as interferon-gamma, whereas Th2 cells produce cytokines such as interleukin (IL)-4, IL-5, and IL-13.[16] Th2 cell secretions, especially IL-5, are responsible for IgA production. Moreover, Treg cells are defined by their ability to produce transforming growth factor-β (TGF-β) which also induces IgA production and inhibits Th2 cell development.[17],[18] Regarding the results of this study, it seems that up to age 40 years, the Th2 cell responses are responsible for salivary IgA production. Hyperactivation of Treg cells that may occur after age 40 years probably results in higher salivary IgA concentrations through the production of TGF-β. Consistent with our observations, an enhancement in Th2 cell responses and also an elevation in the percentages of Treg cells have been described during aging.[19]


   Conclusion Top


The results of the present study demonstrated the profound age-related alterations in salivary IgA levels in healthy individuals. These results encourage further studies to elucidate the precise cellular and molecular mechanisms responsible for these changes to improve oral immunity

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest



 
   References Top

1.
Marcotte H, Lavoie MC. Oral microbial ecology and the role of salivary immunoglobulin A. Microbiol Mol Biol Rev 1998;62:71-109.  Back to cited text no. 1
    
2.
Yamaguchi T. Human salivary aggregation in Streptococcus intermedius type g strains: Relationship with IgA. FEMS Immunol Med Microbiol 2004;41:101-7.  Back to cited text no. 2
    
3.
Fontana M, Gfell LE, Gregory RL. Characterization of preparations enriched for Streptococcus mutans fimbriae: Salivary immunoglobulin A antibodies in caries-free and caries-active subjects. Clin Diagn Lab Immunol 1995;2:719-5.  Back to cited text no. 3
    
4.
Bratthall D, Serinirach R, Hamberg K, Widerström L. Immunoglobulin A reaction to oral streptococci in saliva of subjects with different combinations of caries and levels of mutans streptococci. Oral Microbiol Immunol 1997;12:212-8.  Back to cited text no. 4
    
5.
Russell MW, Kilian M, Lamm ME. Biological activities of IgA. In: Warren S, editor. Mucosal Immunology. San Diego: Academic Press; 1999. p. 225-40.  Back to cited text no. 5
    
6.
Gregory RL, Kim DE, Kindle JC, Hobbs LC, Lloyd DR. Immunoglobulin-degrading enzymes in localized juvenile periodontitis. J Periodontal Res 1992;27:176-83.  Back to cited text no. 6
    
7.
Koga-Ito CY, Martins CA, Balducci I, Jorge AO. Correlation among mutans streptococci counts, dental caries, and IgA to Streptococcus mutans in saliva. Braz Oral Res 2004;18:350-5.  Back to cited text no. 7
    
8.
Soresi S, Togias A. Mechanisms of action of antiimmunoglobulin E therapy. Allergy Asthma Proc 2006;27(2 Suppl 1):S15-23.  Back to cited text no. 8
    
9.
Haeney MR, Smith DJ, King WF, Taubman MA. Tests for circulating immune complexes. Essentials of Clinical immunology. 2nd ed. Oxford, UK: Blackwell Scientific Publication; 1989. p. 190-202.  Back to cited text no. 9
    
10.
Eliasson L, Birkhed D, Osterberg T, Carlen A. Minor salivary gland secretion rates and immunoglobulin A in adults and the elderly. Eur J Oral Sci 2006;114:494-9.  Back to cited text no. 10
    
11.
Weemaes C, Klasen I, Göertz J, Beldhuis-Valkis M, Olafsson O, Haraldsson A. Development of immunoglobulin A in infancy and childhood. Scand J Immunol 2003;58:642-8.  Back to cited text no. 11
    
12.
Childers NK, Greenleaf C, Li F, Dasanayake AP, Powell WD, Michalek SM. Effect of age on immunoglobulin A subclass distribution in human parotid saliva. Oral Microbiol Immunol 2003;18:298-301.  Back to cited text no. 12
    
13.
Challacombe SJ, Percival RS, Marsh PD. Age-related changes in immunoglobulin isotypes in whole and parotid saliva and serum in healthy individuals. Oral Microbiol Immunol 1995;10:202-7.  Back to cited text no. 13
    
14.
Cole MF, Evans MK, Kirchherr JL, Sheridan MJ, Bowden GH. Study of humoral immunity to commensal oral bacteria in human infants demonstrates the presence of secretory immunoglobulin A antibodies reactive with Actinomyces naeslundii genospecies 1 and 2 ribotypes. Clin Diagn Lab Immunol 2004;11:473-82.  Back to cited text no. 14
    
15.
Woof JM, Kerr MA. The function of immunoglobulin A in immunity. J Pathol 2006;208:270-82.  Back to cited text no. 15
    
16.
Usui T. Transcription factors that regulate helper T cell differentiation. Nihon Rinsho Meneki Gakkai Kaishi 2007;30:419-27.  Back to cited text no. 16
    
17.
Taylor A, Verhagen J, Blaser K, Akdis M, Akdis CA. Mechanisms of immune suppression by interleukin-10 and transforming growth factor-beta: The role of T regulatory cells. Immunology 2006;117:433-42.  Back to cited text no. 17
    
18.
Scherf W, Burdach S, Hansen G. Reduced expression of transforming growth factor beta 1 exacerbates pathology in an experimental asthma model. Eur J Immunol 2005;35:198-206.  Back to cited text no. 18
    
19.
Dejaco C, Duftner C, Schirmer M. Are regulatory Tcells linked with aging? Exp Gerontol 2006;41:339-45.  Back to cited text no. 19
    



 
 
    Tables

  [Table 1], [Table 2]



 

Top
 
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

 
  In this article
    Abstract
   Introduction
    Materials and Me...
   Results
   Discussion
   Conclusion
    References
    Article Tables

 Article Access Statistics
    Viewed214    
    Printed4    
    Emailed0    
    PDF Downloaded12    
    Comments [Add]    

Recommend this journal