Journal of Pharmacy And Bioallied Sciences

ORIGINAL ARTICLE
Year
: 2010  |  Volume : 2  |  Issue : 2  |  Page : 105--108

Determination of gallic acid in Phyllanthus emblica Linn. dried fruit powder by HPTLC


Laxman Sawant, Nancy Pandita, Bala Prabhakar 
 School of Pharmacy & Technology Management, SVKM's Narsee Monjee Institute of Management and Higher Studies, V.L.Mehta Road, Vile Parle (west) Mumbai, India

Correspondence Address:
Laxman Sawant
School of Pharmacy & Technology Management, SVKMSQs Narsee Monjee Institute of Management and Higher Studies, V.L.Mehta Road, Vile Parle (west) Mumbai
India

Objective : Emblica (Phyllanthus emblica L.), an euphorbiaceous plant, is widely distributed in subtropical and tropical areas of India, China and Indonesia. The fruits possess antimicrobial, antioxidant, anti-inflammatory, analgesic and antipyretic properties. In the current article a new, simple, sensitive, selective, precise, and robust high-performance thin-layer chromatographic (HPTLC) method was developed and validated for the determination of gallic acid in dried fruit powder of Phyllanthus emblica. Materials and Methods : The quantitative determination of gallic acid was performed on TLC aluminium plates pre-coated with silica gel 60F-254 as the stationary phase. The linear ascending development was carried out in a twin trough glass chamber saturated with a mobile phase consisting of toluene: ethyl acetate: formic acid: methanol (3:3:0.8:0.2) at room temperature (25 ± 2°C). Camag TLC scanner III was used for spectrodensitometric scanning and analysis, in the absorbance mode, at 278 nm. Results : The linear regression analysis data for the calibration plots showed good linear relationship with r 2 = 0.99977 in the concentration range of 40 - 240 ng spot−1, with respect to the peak area. According to the guidelines of the International Conference on Harmonization (ICH), the method was validated for precision, accuracy, and recovery. Conclusion : Statistical analysis of the data showed that the method was reproducible and selective for the estimation of gallic acid.


How to cite this article:
Sawant L, Pandita N, Prabhakar B. Determination of gallic acid in Phyllanthus emblica Linn. dried fruit powder by HPTLC.J Pharm Bioall Sci 2010;2:105-108


How to cite this URL:
Sawant L, Pandita N, Prabhakar B. Determination of gallic acid in Phyllanthus emblica Linn. dried fruit powder by HPTLC. J Pharm Bioall Sci [serial online] 2010 [cited 2021 Jan 26 ];2:105-108
Available from: https://www.jpbsonline.org/article.asp?issn=0975-7406;year=2010;volume=2;issue=2;spage=105;epage=108;aulast=Sawant;type=0