Journal of Pharmacy And Bioallied Sciences

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 7  |  Issue : 3  |  Page : 195--200

Physiologically active hydrogel (in situ gel) of sparfloxacin and its evaluation for ocular retention using gamma scintigraphy


Himanshu Gupta1, Aqil Malik2, RK Khar2, Asgar Ali2, Aseem Bhatnagar4, Gaurav Mittal4 
1 Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard; Department of Nuclear Medicine, Institute of Nuclear Medicine and Allied Sciences, Ministry of Defence, New Delhi, India
2 Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India

Correspondence Address:
Aqil Malik
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi
India

Objective: Due to the structure and physiological barrier of eye, only 1% of instilled dose is available for action on the corneal surface. In this work, we developed and evaluated chitosan (pH sensitive) and gellan gum (ion sensitive) in situ gel of sparfloxacin to improve precorneal residence time. Materials and Methods: A protocol for radiolabeling of sparfloxacin with Tc-99m was optimized to study the ocular retention using gamma scintigraphy technique. Results: The clear formulation was developed. In vitro release showed a sustained and prolonged release compared to plain eye drop solution. Dynamic and static gamma scintigraphy showed better retention than plain eye drops. The ocular tolerance test (hen«SQ»s egg test-chorioallantoic membrane test and infra-red study) showed that the formulation is nonirritant and can be used as ocular vehicle. Conclusion: Radiolabel protocol for sparfloxacin was successfully developed and evaluated on ocular retention studies of developed in situ gel. The developed in situ gel is non irritant and can go further with clinical evaluation.


How to cite this article:
Gupta H, Malik A, Khar R K, Ali A, Bhatnagar A, Mittal G. Physiologically active hydrogel (in situ gel) of sparfloxacin and its evaluation for ocular retention using gamma scintigraphy.J Pharm Bioall Sci 2015;7:195-200


How to cite this URL:
Gupta H, Malik A, Khar R K, Ali A, Bhatnagar A, Mittal G. Physiologically active hydrogel (in situ gel) of sparfloxacin and its evaluation for ocular retention using gamma scintigraphy. J Pharm Bioall Sci [serial online] 2015 [cited 2021 Jun 18 ];7:195-200
Available from: https://www.jpbsonline.org/article.asp?issn=0975-7406;year=2015;volume=7;issue=3;spage=195;epage=200;aulast=Gupta;type=0