Journal of Pharmacy And Bioallied Sciences

SYMPOSIUM - HERBAL DRUGS AND BOTANICALS - RESEARCH ARTICLES
Year
: 2015  |  Volume : 7  |  Issue : 4  |  Page : 260--263

A high performance thin layer chromatographic method for the estimation of colchicine in different formulations


Mohd Fahim1, Mhaveer Singh2, YT Kamal2, Hayat M Mukhtar3, Sayeed Ahmad2 
1 Nandini Nagar College of Pharmacy, Nawabganj, Gonda, Uttar Pradesh, India
2 Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Bioactive Natural Product Laboratory, Hamdard University, New Delhi, India
3 SBS College of Pharmacy, Patti, Punjab, India

Correspondence Address:
Sayeed Ahmad
Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Bioactive Natural Product Laboratory, Hamdard University, New Delhi
India

Background: Colchicine is a main alkaloid present in bitter and sweet variety of colchicum (Colchicum luteum Baker), which have been reported to possess anti-rheumatic, anti-gout, and anticancer potential. Colchicum is an important ingredient of several Unani and Herbal formulations. Quantification of colchicine will play a great role in quality control of these formulations. Hence, a high-performance thin layer chromatographic (TLC) method has been developed for the analysis of colchicine in Unani formulations of various dosage forms such as hubb (tablet) and capsules. Materials and Methods: The samples were applied on aluminum TLC plates precoated with silica gel 60-F254and developed using mobile phase toluene-dichloromethane-methanol in equal proportions. Quantification was done by densitometric scanning at 350 nm, which showed a linear response in the range of 50–500 ng/spot. The developed method was validated as per the International Conference on Harmonization guidelines for linearity, precision, accuracy, specificity, robustness, limit of detection, and limit of quantification. Results and Conclusion: The developed method was applied for quantitative estimation of colchicine in different Unani and Herbal formulations. The method was found simple, selective, accurate with a wide range of linearity, hence suitable for the quality control of different formulations and varieties of colchicum with respect to colchicine content.


How to cite this article:
Fahim M, Singh M, Kamal Y T, Mukhtar HM, Ahmad S. A high performance thin layer chromatographic method for the estimation of colchicine in different formulations.J Pharm Bioall Sci 2015;7:260-263


How to cite this URL:
Fahim M, Singh M, Kamal Y T, Mukhtar HM, Ahmad S. A high performance thin layer chromatographic method for the estimation of colchicine in different formulations. J Pharm Bioall Sci [serial online] 2015 [cited 2020 Nov 28 ];7:260-263
Available from: https://www.jpbsonline.org/article.asp?issn=0975-7406;year=2015;volume=7;issue=4;spage=260;epage=263;aulast=Fahim;type=0