Journal of Pharmacy And Bioallied Sciences

SYMPOSIUM - HERBAL DRUGS AND BOTANICALS - RESEARCH ARTICLES
Year
: 2015  |  Volume : 7  |  Issue : 4  |  Page : 284--288

Characterization of Linum usitatissimum L. oil obtained from different extraction technique and in vitro antioxidant potential of supercritical fluid extract


Rishika Chauhan1, Karishma Chester2, Yasmeen Khan1, Ennus Tajuddin Tamboli1, Sayeed Ahmad1 
1 Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard University, New Delhi, India
2 Department of Pharmacy, Bansthali Vidyapith, Bansthali University, Tonk, Rajasthan, India

Correspondence Address:
Sayeed Ahmad
Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard University, New Delhi
India

Aim: Present investigation was aimed to characterize the fixed oil of Linum usitatissimum L. using five different extraction methods: Supercritical fluid extraction (SFE), ultrasound-assistance, soxhlet extraction, solvent extraction, and three phase partitioning method. Materials and Methods: The SFE conditions (temperature, pressure, and volume of CO2) were optimized prior for better yield. The extracted oils were analyzed and compared for their physiochemical parameters, high performance thin layer chromatography (HPTLC), gas chromatography-mass spectrometry (GC-MS), and Fourier-transformed infrared spectroscopy (FT-IR) fingerprinting. Antioxidant activity was also determined using 1,1-diphenyl-2-picrylhydrazyl and superoxide scavenging method. Result: The main fatty acids were α-linolenic acid, linoleic acid, palmitic acid, and stearic acid as obtained by GC-MS. HPTLC analysis revealed the presence of similar major components in chromatograms. Similarly, the pattern of peaks, as obtained in FT-IR and GC-MS spectra of same oils by different extraction methods, were superimposable. Conclusion: Analysis reported that the fixed oil of L. usitatissimum L. is a good source of n-3 fatty acid with the significant antioxidant activity of oil obtained from SFE extraction method.


How to cite this article:
Chauhan R, Chester K, Khan Y, Tamboli ET, Ahmad S. Characterization of Linum usitatissimum L. oil obtained from different extraction technique and in vitro antioxidant potential of supercritical fluid extract.J Pharm Bioall Sci 2015;7:284-288


How to cite this URL:
Chauhan R, Chester K, Khan Y, Tamboli ET, Ahmad S. Characterization of Linum usitatissimum L. oil obtained from different extraction technique and in vitro antioxidant potential of supercritical fluid extract. J Pharm Bioall Sci [serial online] 2015 [cited 2020 Dec 4 ];7:284-288
Available from: https://www.jpbsonline.org/article.asp?issn=0975-7406;year=2015;volume=7;issue=4;spage=284;epage=288;aulast=Chauhan;type=0