Journal of Pharmacy And Bioallied Sciences

SYMPOSIUM - HERBAL DRUGS AND BOTANICALS - RESEARCH ARTICLES
Year
: 2015  |  Volume : 7  |  Issue : 4  |  Page : 304--307

Optimization of ultrasound-assisted extraction of charantin from Momordica charantia fruits using response surface methodology


Javed Ahamad1, Saima Amin2, Showkat R Mir1 
1 Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Hamdard University, PO Hamdard Nagar, New Delhi, India
2 Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, PO Hamdard Nagar, New Delhi, India

Correspondence Address:
Showkat R Mir
Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Hamdard University, PO Hamdard Nagar, New Delhi
India

Background: Momordica charantia Linn. (Cucurbitaceae) fruits are well known for their beneficial effects in diabetes that are often attributed to its bioactive component charantin. Objective: The aim of the present study is to develop and optimize an efficient protocol for the extraction of charantin from M. charantia fruits. Materials and Methods: Response surface methodology (RSM) was used for the optimization of ultrasound-assisted extraction (UAE) conditions. RSM was based on a three-level, three-variable Box-Behnken design (BBD), and the studied variables included solid to solvent ratio, extraction temperature, and extraction time. Results: The optimal conditions predicted by the BBD were: UAE with methanol: Water (80:20, v/v) at 46°C for 120 min with solid to solvent ratio of 1:26 w/v, under which the yield of charantin was 3.18 mg/g. Confirmation trials under slightly adjusted conditions yielded 3.12 ± 0.14 mg/g of charantin on dry weight basis of fruits. The result of UAE was also compared with Soxhlet extraction method and UAE was found 2.74-fold more efficient than the Soxhlet extraction for extracting charantin. Conclusions:A facile UAE protocol for a high extraction yield of charantin was developed and validated.


How to cite this article:
Ahamad J, Amin S, Mir SR. Optimization of ultrasound-assisted extraction of charantin from Momordica charantia fruits using response surface methodology.J Pharm Bioall Sci 2015;7:304-307


How to cite this URL:
Ahamad J, Amin S, Mir SR. Optimization of ultrasound-assisted extraction of charantin from Momordica charantia fruits using response surface methodology. J Pharm Bioall Sci [serial online] 2015 [cited 2020 Nov 28 ];7:304-307
Available from: https://www.jpbsonline.org/article.asp?issn=0975-7406;year=2015;volume=7;issue=4;spage=304;epage=307;aulast=Ahamad;type=0