Journal of Pharmacy And Bioallied Sciences

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 12  |  Issue : 5  |  Page : 274--278

An immunohistochemical study showing Ki-67 as an analytical marker in oral malignant and premalignant lesions


Kailash C Dash, Niva Mahapatra, Lipsa Bhuyan, Abikshyeet Panda, Shyam S Behura, Pallavi Mishra 
 Department of Oral Pathology and Microbiology, Kalinga Institute of Dental Sciences, Kalinga Institute of Industrial Technology, Deemed to be University, Bhubaneswar, Odisha, India

Correspondence Address:
Niva Mahapatra
Department of Oral Pathology and Microbiology, Kalinga Institute of Dental Sciences, Kalinga Institute of Industrial Technology Deemed to be University, Bhubaneswar, Odisha.
India

Introduction: Ki-67 is a nuclear protein. It is a proliferation marker that has an essential function in tumorigenesis due to its positive connection with tumor expansion. Aim: The aim of this study was to evaluate the articulation of Ki‑67 as prognostic marker in various grades of oral epithelial dysplasia (OED) and in oral squamous cell carcinoma (OSCC). Materials and Methods: A total of 100 histologically affirmed samples of normal oral mucosa (NOM), OED, and OSCC were divided into three groups—Group I (10 samples of normal oral mucosa), Group II (45 samples of OED), Group III (45 samples of OSCC). Routine hematoxylin and eosin and immunohistochemical staining with Ki-67 monoclonal antibody were carried out in all the samples. Results: Within Group I, articulation of Ki-67 was constrained to the basal layers. In Group II, cells showing positive expression of Ki-67 were available in the basal, suprabasal, and spinous layers. Cells showing positive expression of Ki-67 among well-differentiated OSCC were presented mainly in the periphery of the tumor nests; in moderately differentiated OSCC, cells were located in both peripheral and part of a center of the tumor nests; and in most cases of poorly differentiated OSCC, cells were diffused. Statistically significant difference in positive expression of Ki-67 was appreciated between three groups. Conclusion: Ki-67 antigen may perhaps be used as a marker for the histological reviewing of OED and OSCC. With the increase in the severity of OED, cells showing positive expression of Ki-67 also increased.


How to cite this article:
Dash KC, Mahapatra N, Bhuyan L, Panda A, Behura SS, Mishra P. An immunohistochemical study showing Ki-67 as an analytical marker in oral malignant and premalignant lesions.J Pharm Bioall Sci 2020;12:274-278


How to cite this URL:
Dash KC, Mahapatra N, Bhuyan L, Panda A, Behura SS, Mishra P. An immunohistochemical study showing Ki-67 as an analytical marker in oral malignant and premalignant lesions. J Pharm Bioall Sci [serial online] 2020 [cited 2020 Oct 26 ];12:274-278
Available from: https://www.jpbsonline.org/article.asp?issn=0975-7406;year=2020;volume=12;issue=5;spage=274;epage=278;aulast=Dash;type=0