Journal of Pharmacy And Bioallied Sciences

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 12  |  Issue : 5  |  Page : 448--452

Comparison of antibacterial effect of calcium hydroxide combined with chlorhexidine and povidone-iodine against Enterococcus faecalis in dentinal tubules of human incisors: An in vitro comparative study


Sameer Punathil1, Shabna Moyin2, Sham S Bhat3, Sundeep Hedge3, Archana Pai1, Jeswin James1 
1 Department of Pediatric and Preventive Dentistry, Sree Anjaneya Institute of Dental Sciences, Calicut, Kerala, India
2 Department of Conservative Dentistry and Endodontics, Sree Anjaneya Institute of Dental Sciences, Calicut, Kerala, India
3 Department of Pediatric and Preventive Dentistry, Yenepoya Dental College, Mangalore, Karnataka, India

Correspondence Address:
Sameer Punathil
Department of Pediatric and Preventive Dentistry, Sree Anjaneya Institute of Dental Sciences, Calicut, Kerala.
India

Background and Objectives: It has been found that the microorganism behind the failure of root-filled teeth is Enterococcus faecalis, which shows resistance to most of the intra-canal medicaments. Therefore, the purpose of this study was to evaluate the antibacterial efficacy of three intra-canal medications—calcium hydroxide with saline, combinations of calcium hydroxide with 2% chlorhexidine (CHX), and calcium hydroxide with 5% povidone-iodine—against E. faecalis in dentinal tubules of human incisors. Materials and Methods: Forty permanent maxillary central incisors were made into standardized segments and infected with E. faecalis. They were treated with a paste made of calcium hydroxide and 2% CHX, calcium hydroxide and 5% povidone-iodine, and calcium hydroxide and saline for 1 week. Dentinal shavings collected from the canal were suspended in thioglycollate broth solution and spread on brain heart infusion agar. Colony-forming units (CFUs) were enumerated and the CFU per milligram of dentin was calculated. The pH of the medicaments used was measured with the help of pH meter. Results: The results showed that the paste made from calcium hydroxide and 2% CHX was significantly more effective than that made from calcium hydroxide and povidone-iodine, and calcium hydroxide and saline. The addition of CHX or povidone-iodine did not affect the alkalinity of calcium hydroxide. Conclusion: This study concludes that Ca(OH)2 + 2% CHX are effective against E. faecalis. Combinations of calcium hydroxide and 5% povidone-iodine showed better antibacterial effect than calcium hydroxide and saline. Ca(OH)2 + saline was ineffective against E. faecalis.


How to cite this article:
Punathil S, Moyin S, Bhat SS, Hedge S, Pai A, James J. Comparison of antibacterial effect of calcium hydroxide combined with chlorhexidine and povidone-iodine against Enterococcus faecalis in dentinal tubules of human incisors: An in vitro comparative study.J Pharm Bioall Sci 2020;12:448-452


How to cite this URL:
Punathil S, Moyin S, Bhat SS, Hedge S, Pai A, James J. Comparison of antibacterial effect of calcium hydroxide combined with chlorhexidine and povidone-iodine against Enterococcus faecalis in dentinal tubules of human incisors: An in vitro comparative study. J Pharm Bioall Sci [serial online] 2020 [cited 2020 Oct 27 ];12:448-452
Available from: https://www.jpbsonline.org/article.asp?issn=0975-7406;year=2020;volume=12;issue=5;spage=448;epage=452;aulast=Punathil;type=0