Journal of Pharmacy And Bioallied Sciences

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 12  |  Issue : 5  |  Page : 607--612

Assessment of quality of platelet-rich plasma produced with desktop centrifuge and comparison with standardized commercially available Platelet-rich plasma


V Ramesh Srinivasan1, M Rekha1, Effie Edsor1, S Pradheep Raja1, T Dinesh Kumar2, S Kalaiselvan3 
1 Department of Oral and Maxillofacial Surgery, RVS Dental College and Hospital, Coimbatore, Tamil Nadu, India
2 Department of Oral and Maxillofacial Pathology, RVS Dental College and Hospital, Coimbatore, Tamil Nadu, India
3 Department of Oral and Maxillofacial Surgery, CSI Dental College, Madurai, Tamil Nadu, India

Correspondence Address:
V Ramesh Srinivasan
RVS Dental College and Hospital, Trichy Road, Kannampalayam, Sulur, Coimbatore, Tamil Nadu.
India

Aims: The aim of this study was to assess the quality of platelet-rich plasma (PRP) produced by in-house desktop centrifuge method and compare it with that of standardized commercial PRP. Materials and Methods: REMI desktop centrifuge was used to prepare PRP and to compare with standardized commercial PRP by calculating the quantity of platelets using Beckman Coulter cell counter in 10 PRP samples and assessing the morphological quality of platelets using JEOL JEM transmission electron microscope (TEM). Statistical Analysis Used: The t test for platelet count in desktop PRP with the test value of therapeutic PRP was 12.618. The P value was <0.001, which was significant statistically. The data followed a normal distribution in normal Q-Q plot for platelet count in desktop centrifuge. So the test samples were not much deviated. Results: The platelet count was lesser than that of standardized commercial PRP. When viewed under JEOL JEM transmission electron microscope, the α granules in platelets were intact and the morphological quality of the PRP was good. Conclusions: With this study, we have determined that the morphological quality of PRP produced by the in-house desktop centrifuge method is comparable to that of standardized commercial PRP. Though the quantity of platelets was less than 1 million cells/μL, the clinical results were good with desired bone formation, thereby providing good avenue for further research.


How to cite this article:
Srinivasan V R, Rekha M, Edsor E, Raja S P, Kumar T D, Kalaiselvan S. Assessment of quality of platelet-rich plasma produced with desktop centrifuge and comparison with standardized commercially available Platelet-rich plasma.J Pharm Bioall Sci 2020;12:607-612


How to cite this URL:
Srinivasan V R, Rekha M, Edsor E, Raja S P, Kumar T D, Kalaiselvan S. Assessment of quality of platelet-rich plasma produced with desktop centrifuge and comparison with standardized commercially available Platelet-rich plasma. J Pharm Bioall Sci [serial online] 2020 [cited 2020 Oct 25 ];12:607-612
Available from: https://www.jpbsonline.org/article.asp?issn=0975-7406;year=2020;volume=12;issue=5;spage=607;epage=612;aulast=Srinivasan;type=0