Journal of Pharmacy And Bioallied Sciences

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 12  |  Issue : 5  |  Page : 73--77

Comparative evaluation of pH and in vitro cytotoxicity of zinc oxide–ozonated eugenol and conventional zinc oxide eugenol as endodontic sealers


Chandramohan Ravivarman1, Athikesavan Jeyasenthil2, Ranganathan Ajay3, Nasir Nilofernisha4, Radhakrishnan Karthikeyan5, Durairaj Rajkumar6 
1 Department of Conservative Dentistry & Endodontics, Dhanalakshmi Srinivasan Medical College & Hospital, Perambalur, Tamil Nadu, India
2 Department of Conservative Dentistry & Endodontics, Priyadarshini Dental College & Hospital, Chennai, Tamil Nadu, India
3 Department of Prosthodontics and Crown & Bridge, Vivekanandha Dental College for Women, Tiruchengode, Tamil Nadu, India
4 Department of Conservative Dentistry & Endodontics, Mahsa University, Kuala Lumpur, Malaysia
5 Department of Pedodontics and Preventive Dentistry, Sri Ramakrishna Dental College & Hospital, Coimbatore, Tamil Nadu, India
6 Department of Conservative Dentistry & Endodontics, Private Practitioner, Tanjore, Tamil Nadu, India

Correspondence Address:
Chandramohan Ravivarman
Department of Conservative Dentistry & Endodontics, Dhanalakshmi Srinivasan Medical College & Hospital, Tamil Nadu.
India

Background: Eugenol released from zinc oxide eugenol (ZOE)–based sealants may cause irritation to the periapical tissues and has cytotoxic potential. Ozone therapy has numerous clinical applications with humans because of its bactericidal action, detoxifying effect, stimulation of angiogenesis, and wound-healing capacity. Therefore ozone can be incorporated in ZOE sealer to exploit these properties. Materials and Methods: Eugenol was ozonated using ozonator machine and the samples were divided into two groups: Group I: zinc oxide eugenol (n = 10) and Group II: zinc oxide–ozonated eugenol (OZOE; n = 10). The pH of the fresh sealer samples and the set samples was measured using calibrated pH meter after predetermined time intervals. Cytotoxicity of the set sealer was evaluated on mouse L929 fibroblasts using cellular metabolic assay. Results: pH of the samples in Group II was higher when compared to Group I. Group II showed higher cell viability than the Group I. Conclusion: OZOE sealers can be used as an alternative to the conventional ZOE sealers.


How to cite this article:
Ravivarman C, Jeyasenthil A, Ajay R, Nilofernisha N, Karthikeyan R, Rajkumar D. Comparative evaluation of pH and in vitro cytotoxicity of zinc oxide–ozonated eugenol and conventional zinc oxide eugenol as endodontic sealers.J Pharm Bioall Sci 2020;12:73-77


How to cite this URL:
Ravivarman C, Jeyasenthil A, Ajay R, Nilofernisha N, Karthikeyan R, Rajkumar D. Comparative evaluation of pH and in vitro cytotoxicity of zinc oxide–ozonated eugenol and conventional zinc oxide eugenol as endodontic sealers. J Pharm Bioall Sci [serial online] 2020 [cited 2021 Apr 14 ];12:73-77
Available from: https://www.jpbsonline.org/article.asp?issn=0975-7406;year=2020;volume=12;issue=5;spage=73;epage=77;aulast=Ravivarman;type=0