Journal of Pharmacy And Bioallied Sciences

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 12  |  Issue : 6  |  Page : 831--835

Effect of non-hydrogen peroxide on antibacterial activity of Malaysian Meliponini honey against Staphylococcus aureus


Fatima Ibrahim Jibril1, Abu Bakar Mohd Hilmi1, Sani Aliyu2 
1 School of Biomedicine, Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Terengganu, Malaysia
2 Department of Microbiology, Umaru Musa Yar’adua University Katsina, Katsina, Nigeria

Correspondence Address:
Dr. Abu Bakar Mohd Hilmi
School of Biomedicine, Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Kuala Nerus, Terengganu.
Malaysia

Introduction: Stingless bee is an insect that belongs to the family Apidae. Its name is based on its disability of stinging. It has a high product of Meliponini honey and propolis by which are commonly referred to as stingless bee honey and stingless bee propolis. Meliponini honey is one of the crucial natural sources and has the potential to kill infectious microorganisms. Previous studies have proved that the antibacterial activity of natural honey was an effect of hydrogen peroxide, a substance contained in the honey. However, these claims were contradicting with too many studies. Objective: Therefore, this study aimed to identify the antibacterial activity of Malaysian Meliponini honey which contained non-hydrogen peroxide against Staphylococcus aureus, an opportunistic microbial. Materials and Methods: Meliponini honey was used as an antibacterial agent for the treatment of S. aureus in agar well diffusion assay. An amplex red hydrogen peroxide kit was used to identify the hydrogen peroxide in the honey sample. Meanwhile, non-hydrogen peroxide activity was performed by using honey-catalase treated. Results: For the first time, we found that hydrogen peroxide was absent in all Meliponini honey samples. Meliponini honey has higher antibacterial activity (13.30 ± 0.56 mm) compared to Apis honey (9.03 ± 0.22 mm) in agar well diffusion assay. Discussion: Non-hydrogen peroxide in Meliponini honey is a bioactive compound and beneficial to kill the microbial infection. Conclusion: Antibacterial activity of Malaysian Meliponini honey is directly contributed by non-hydrogen peroxide.


How to cite this article:
Jibril FI, Mohd Hilmi AB, Aliyu S. Effect of non-hydrogen peroxide on antibacterial activity of Malaysian Meliponini honey against Staphylococcus aureus.J Pharm Bioall Sci 2020;12:831-835


How to cite this URL:
Jibril FI, Mohd Hilmi AB, Aliyu S. Effect of non-hydrogen peroxide on antibacterial activity of Malaysian Meliponini honey against Staphylococcus aureus. J Pharm Bioall Sci [serial online] 2020 [cited 2022 Aug 11 ];12:831-835
Available from: https://www.jpbsonline.org/article.asp?issn=0975-7406;year=2020;volume=12;issue=6;spage=831;epage=835;aulast=Jibril;type=0